Captopril (an inhibitor of angiotensin converting enzyme) inhibits obstructive changes in the neonatal rabbit bladder

Earl Y Cheng*, Chung Lee, Robert S. Decker, Julia A. Sensibar, Sharon Lang, William E. Kaplan, Max Maizels, Casimir F. Firlit

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objectives. To investigate whether angiotensin II has a role in the regulation of bladder smooth muscle growth and function, we developed a model of bladder neck obstruction (BNO) in the neonatal rabbit and investigated the effect of captopril (angiotensin converting enzyme inhibitor) on the obstructive changes in the developing bladder. Methods. Partial BNO was induced in a group of 2-day-old rabbits (n = 8) by placing a loose 2-0 silk ligature around the vesicourethral junction. A second group of rabbits subjected to the identical partial BNO procedure (n = 8) was given captopril (1 mg/kg/day). Twelve days postobstruction, bladders from these animals, along with paired controls (n = 8), were harvested and assayed for total protein, DNA, and collagen content. Results. Partial BNO resulted in a 170% increase in wet weight (P <0.05), 132% increase in protein/deoxyribonucleic acid (DNA) ratio (P <0.05), 75% increase in total DNA (P <0.05), and 115% increase in total collagen (P <0.05). When compared with obstructed animals, captopril administration significantly inhibited the increase in total DNA (P <0.05) and reduced the amount of total collagen (P = 0.054). Examination of histology specimens demonstrated that captopril inhibited the serosal hyperplasia and collagen deposition associated with obstruction. Conclusions. These data demonstrate that captopril partially inhibits the changes in the neonatal rabbit bladder associated with obstruction, supporting the hypothesis that angiotensin II is involved in the regulation of bladder smooth muscle growth and collagen production.

Original languageEnglish (US)
Pages (from-to)465-471
Number of pages7
JournalUrology
Volume50
Issue number3
DOIs
StatePublished - Sep 1 1997

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Captopril
Urinary Bladder Neck Obstruction
Angiotensin-Converting Enzyme Inhibitors
Urinary Bladder
Collagen
Rabbits
DNA
Angiotensin II
Smooth Muscle
Silk
Growth
Hyperplasia
Ligation
Histology
Proteins
Weights and Measures

ASJC Scopus subject areas

  • Urology

Cite this

Cheng, Earl Y ; Lee, Chung ; Decker, Robert S. ; Sensibar, Julia A. ; Lang, Sharon ; Kaplan, William E. ; Maizels, Max ; Firlit, Casimir F. / Captopril (an inhibitor of angiotensin converting enzyme) inhibits obstructive changes in the neonatal rabbit bladder. In: Urology. 1997 ; Vol. 50, No. 3. pp. 465-471.
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abstract = "Objectives. To investigate whether angiotensin II has a role in the regulation of bladder smooth muscle growth and function, we developed a model of bladder neck obstruction (BNO) in the neonatal rabbit and investigated the effect of captopril (angiotensin converting enzyme inhibitor) on the obstructive changes in the developing bladder. Methods. Partial BNO was induced in a group of 2-day-old rabbits (n = 8) by placing a loose 2-0 silk ligature around the vesicourethral junction. A second group of rabbits subjected to the identical partial BNO procedure (n = 8) was given captopril (1 mg/kg/day). Twelve days postobstruction, bladders from these animals, along with paired controls (n = 8), were harvested and assayed for total protein, DNA, and collagen content. Results. Partial BNO resulted in a 170{\%} increase in wet weight (P <0.05), 132{\%} increase in protein/deoxyribonucleic acid (DNA) ratio (P <0.05), 75{\%} increase in total DNA (P <0.05), and 115{\%} increase in total collagen (P <0.05). When compared with obstructed animals, captopril administration significantly inhibited the increase in total DNA (P <0.05) and reduced the amount of total collagen (P = 0.054). Examination of histology specimens demonstrated that captopril inhibited the serosal hyperplasia and collagen deposition associated with obstruction. Conclusions. These data demonstrate that captopril partially inhibits the changes in the neonatal rabbit bladder associated with obstruction, supporting the hypothesis that angiotensin II is involved in the regulation of bladder smooth muscle growth and collagen production.",
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Captopril (an inhibitor of angiotensin converting enzyme) inhibits obstructive changes in the neonatal rabbit bladder. / Cheng, Earl Y; Lee, Chung; Decker, Robert S.; Sensibar, Julia A.; Lang, Sharon; Kaplan, William E.; Maizels, Max; Firlit, Casimir F.

In: Urology, Vol. 50, No. 3, 01.09.1997, p. 465-471.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Captopril (an inhibitor of angiotensin converting enzyme) inhibits obstructive changes in the neonatal rabbit bladder

AU - Cheng, Earl Y

AU - Lee, Chung

AU - Decker, Robert S.

AU - Sensibar, Julia A.

AU - Lang, Sharon

AU - Kaplan, William E.

AU - Maizels, Max

AU - Firlit, Casimir F.

PY - 1997/9/1

Y1 - 1997/9/1

N2 - Objectives. To investigate whether angiotensin II has a role in the regulation of bladder smooth muscle growth and function, we developed a model of bladder neck obstruction (BNO) in the neonatal rabbit and investigated the effect of captopril (angiotensin converting enzyme inhibitor) on the obstructive changes in the developing bladder. Methods. Partial BNO was induced in a group of 2-day-old rabbits (n = 8) by placing a loose 2-0 silk ligature around the vesicourethral junction. A second group of rabbits subjected to the identical partial BNO procedure (n = 8) was given captopril (1 mg/kg/day). Twelve days postobstruction, bladders from these animals, along with paired controls (n = 8), were harvested and assayed for total protein, DNA, and collagen content. Results. Partial BNO resulted in a 170% increase in wet weight (P <0.05), 132% increase in protein/deoxyribonucleic acid (DNA) ratio (P <0.05), 75% increase in total DNA (P <0.05), and 115% increase in total collagen (P <0.05). When compared with obstructed animals, captopril administration significantly inhibited the increase in total DNA (P <0.05) and reduced the amount of total collagen (P = 0.054). Examination of histology specimens demonstrated that captopril inhibited the serosal hyperplasia and collagen deposition associated with obstruction. Conclusions. These data demonstrate that captopril partially inhibits the changes in the neonatal rabbit bladder associated with obstruction, supporting the hypothesis that angiotensin II is involved in the regulation of bladder smooth muscle growth and collagen production.

AB - Objectives. To investigate whether angiotensin II has a role in the regulation of bladder smooth muscle growth and function, we developed a model of bladder neck obstruction (BNO) in the neonatal rabbit and investigated the effect of captopril (angiotensin converting enzyme inhibitor) on the obstructive changes in the developing bladder. Methods. Partial BNO was induced in a group of 2-day-old rabbits (n = 8) by placing a loose 2-0 silk ligature around the vesicourethral junction. A second group of rabbits subjected to the identical partial BNO procedure (n = 8) was given captopril (1 mg/kg/day). Twelve days postobstruction, bladders from these animals, along with paired controls (n = 8), were harvested and assayed for total protein, DNA, and collagen content. Results. Partial BNO resulted in a 170% increase in wet weight (P <0.05), 132% increase in protein/deoxyribonucleic acid (DNA) ratio (P <0.05), 75% increase in total DNA (P <0.05), and 115% increase in total collagen (P <0.05). When compared with obstructed animals, captopril administration significantly inhibited the increase in total DNA (P <0.05) and reduced the amount of total collagen (P = 0.054). Examination of histology specimens demonstrated that captopril inhibited the serosal hyperplasia and collagen deposition associated with obstruction. Conclusions. These data demonstrate that captopril partially inhibits the changes in the neonatal rabbit bladder associated with obstruction, supporting the hypothesis that angiotensin II is involved in the regulation of bladder smooth muscle growth and collagen production.

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