Abstract
Pancreatic acinar carcinoma fragments, pulse-chase labeled with [3H]-leucine, responded to carbamylcholine chloride by increased secretion of [3H]leucine-labeled protein into external buffer medium. Secretion of labeled protein by the carcinoma fragments increased in concentration-dependent fashion between 10-8-10-5 M carbamylcholine, was completely inhibited at 4°C, and was accompanied by an equivalent increase in the secretion of preformed amylase. A maximally effective carbamylcholine concentration of 10-5 M was observed for both carcinoma fragments and normal pancreas lobules. However, the maximal rate of protein secretion by the carcinoma fragments was only approximately one-fifth the rate determined for the normal pancreas lobules. This modest secretory response indicates a population of partially differentiated cells in the pancreatic acinar carcinoma which secrete less enzyme than fully differentiated adult pancreatic acinar cells. Secretory responsiveness can be utilized as a quantitative acinar cell response in studies on differentiation in pancreatic carcinoma.
Original language | English (US) |
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Pages (from-to) | 245-253 |
Number of pages | 9 |
Journal | Cancer Letters |
Volume | 15 |
Issue number | 3 |
DOIs | |
State | Published - 1982 |
ASJC Scopus subject areas
- Oncology
- Cancer Research