Carbamylcholine stimulation of protein secretion in pancreatic acinar carcinoma of rat

John R. Warren; Michael J. Trump; Janardan K. Reddy; Michael J. Becich

doi:10.1016/0304-3835(82)90125-2

Carbamylcholine stimulation of protein secretion in pancreatic acinar carcinoma of rat

John R. Warren^*, Michael J. Trump, Janardan K. Reddy, Michael J. Becich

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Pancreatic acinar carcinoma fragments, pulse-chase labeled with [³H]-leucine, responded to carbamylcholine chloride by increased secretion of [³H]leucine-labeled protein into external buffer medium. Secretion of labeled protein by the carcinoma fragments increased in concentration-dependent fashion between 10^-8-10^-5 M carbamylcholine, was completely inhibited at 4°C, and was accompanied by an equivalent increase in the secretion of preformed amylase. A maximally effective carbamylcholine concentration of 10^-5 M was observed for both carcinoma fragments and normal pancreas lobules. However, the maximal rate of protein secretion by the carcinoma fragments was only approximately one-fifth the rate determined for the normal pancreas lobules. This modest secretory response indicates a population of partially differentiated cells in the pancreatic acinar carcinoma which secrete less enzyme than fully differentiated adult pancreatic acinar cells. Secretory responsiveness can be utilized as a quantitative acinar cell response in studies on differentiation in pancreatic carcinoma.

Original language	English (US)
Pages (from-to)	245-253
Number of pages	9
Journal	Cancer Letters
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(82)90125-2
State	Published - 1982

ASJC Scopus subject areas

Oncology
Cancer Research

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@article{ef3f11b710e74fffa46e94f066cb9133,

title = "Carbamylcholine stimulation of protein secretion in pancreatic acinar carcinoma of rat",

abstract = "Pancreatic acinar carcinoma fragments, pulse-chase labeled with [3H]-leucine, responded to carbamylcholine chloride by increased secretion of [3H]leucine-labeled protein into external buffer medium. Secretion of labeled protein by the carcinoma fragments increased in concentration-dependent fashion between 10-8-10-5 M carbamylcholine, was completely inhibited at 4°C, and was accompanied by an equivalent increase in the secretion of preformed amylase. A maximally effective carbamylcholine concentration of 10-5 M was observed for both carcinoma fragments and normal pancreas lobules. However, the maximal rate of protein secretion by the carcinoma fragments was only approximately one-fifth the rate determined for the normal pancreas lobules. This modest secretory response indicates a population of partially differentiated cells in the pancreatic acinar carcinoma which secrete less enzyme than fully differentiated adult pancreatic acinar cells. Secretory responsiveness can be utilized as a quantitative acinar cell response in studies on differentiation in pancreatic carcinoma.",

author = "Warren, {John R.} and Trump, {Michael J.} and Reddy, {Janardan K.} and Becich, {Michael J.}",

note = "Funding Information: This work was supported by PHS grants CA 27443 and CA 23055, award ed by the National Cancer Institute, Department of Health and Human Services. We gratefully acknowledge the excellent technical assistance of Rosetta Young Kale. We also thank Lola River0 for secretarial help in preparation of this manuscript.",

year = "1982",

doi = "10.1016/0304-3835(82)90125-2",

language = "English (US)",

volume = "15",

pages = "245--253",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "3",

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T1 - Carbamylcholine stimulation of protein secretion in pancreatic acinar carcinoma of rat

AU - Warren, John R.

AU - Trump, Michael J.

AU - Reddy, Janardan K.

AU - Becich, Michael J.

N1 - Funding Information: This work was supported by PHS grants CA 27443 and CA 23055, award ed by the National Cancer Institute, Department of Health and Human Services. We gratefully acknowledge the excellent technical assistance of Rosetta Young Kale. We also thank Lola River0 for secretarial help in preparation of this manuscript.

PY - 1982

Y1 - 1982

N2 - Pancreatic acinar carcinoma fragments, pulse-chase labeled with [3H]-leucine, responded to carbamylcholine chloride by increased secretion of [3H]leucine-labeled protein into external buffer medium. Secretion of labeled protein by the carcinoma fragments increased in concentration-dependent fashion between 10-8-10-5 M carbamylcholine, was completely inhibited at 4°C, and was accompanied by an equivalent increase in the secretion of preformed amylase. A maximally effective carbamylcholine concentration of 10-5 M was observed for both carcinoma fragments and normal pancreas lobules. However, the maximal rate of protein secretion by the carcinoma fragments was only approximately one-fifth the rate determined for the normal pancreas lobules. This modest secretory response indicates a population of partially differentiated cells in the pancreatic acinar carcinoma which secrete less enzyme than fully differentiated adult pancreatic acinar cells. Secretory responsiveness can be utilized as a quantitative acinar cell response in studies on differentiation in pancreatic carcinoma.

AB - Pancreatic acinar carcinoma fragments, pulse-chase labeled with [3H]-leucine, responded to carbamylcholine chloride by increased secretion of [3H]leucine-labeled protein into external buffer medium. Secretion of labeled protein by the carcinoma fragments increased in concentration-dependent fashion between 10-8-10-5 M carbamylcholine, was completely inhibited at 4°C, and was accompanied by an equivalent increase in the secretion of preformed amylase. A maximally effective carbamylcholine concentration of 10-5 M was observed for both carcinoma fragments and normal pancreas lobules. However, the maximal rate of protein secretion by the carcinoma fragments was only approximately one-fifth the rate determined for the normal pancreas lobules. This modest secretory response indicates a population of partially differentiated cells in the pancreatic acinar carcinoma which secrete less enzyme than fully differentiated adult pancreatic acinar cells. Secretory responsiveness can be utilized as a quantitative acinar cell response in studies on differentiation in pancreatic carcinoma.

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DO - 10.1016/0304-3835(82)90125-2

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AN - SCOPUS:0020030068

VL - 15

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JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 3

ER -

Carbamylcholine stimulation of protein secretion in pancreatic acinar carcinoma of rat

Abstract

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