TY - JOUR
T1 - Carbon isotopic fractionation in heterotrophic microbial metabolism
AU - Blair, N.
AU - Leu, A.
AU - Munoz, E.
AU - Olsen, J.
AU - Kwong, E.
AU - Des Marais, D.
PY - 1985
Y1 - 1985
N2 - Differences in the natural-abundance carbon stable isotopic compositions between products from aerobic cultures of Escherichia coli K-12 were measured. Respired CO2 was 3.4‰ depleted in 13C relative to the glucose used as the carbon source, whereas the acetate was 12.3‰ enriched in 13C. The acetate 13C enrichment was solely in the carboxyl group. Even though the total cellular carbon was only 0.6‰ depleted in 13C, intracellular components exhibited a significant isotopic heterogeneity. The protein and lipid fractions were -1.1 and -2.7‰, respectively. Aspartic and glutamic acids were -1.6 and +2.7‰, respectively, yet citrate was isotopically identical to the glucose. Probable sites of carbon isotopic fractionation include the enzyme, phosphotransacetylase, and the Krebs cycle.
AB - Differences in the natural-abundance carbon stable isotopic compositions between products from aerobic cultures of Escherichia coli K-12 were measured. Respired CO2 was 3.4‰ depleted in 13C relative to the glucose used as the carbon source, whereas the acetate was 12.3‰ enriched in 13C. The acetate 13C enrichment was solely in the carboxyl group. Even though the total cellular carbon was only 0.6‰ depleted in 13C, intracellular components exhibited a significant isotopic heterogeneity. The protein and lipid fractions were -1.1 and -2.7‰, respectively. Aspartic and glutamic acids were -1.6 and +2.7‰, respectively, yet citrate was isotopically identical to the glucose. Probable sites of carbon isotopic fractionation include the enzyme, phosphotransacetylase, and the Krebs cycle.
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U2 - 10.1128/aem.50.4.996-1001.1985
DO - 10.1128/aem.50.4.996-1001.1985
M3 - Article
C2 - 2867741
AN - SCOPUS:0022387770
SN - 0099-2240
VL - 50
SP - 996
EP - 1001
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 4
ER -