Carbonic anhydrase II and alveolar fluid reabsorption during hypercapnia

Jiwang Chen, Emilia Lecuona, Arturo Briva, Lynn C. Welch, Jacob I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Carbonic anhydrase II (CAII) plays an important role in carbon dioxide metabolism and intracellular pH regulation. In this study, we provide evidence that CAII is expressed in both type I (AECI) and type II (AECII) alveolar epithelial cells by RT-PCR and Western blotting in freshly isolated rat cells. These results were further confirmed by double immunostaining with CAII antibodies and AECI- or AECII-specific markers in freshly isolated alveolar epithelial cells and rat lung tissues. Inhibition of CAII by acetazolamide or methazolamide delayed the decrease in the intracellular pH observed during hypercapnia in cultured AECI, AECII, and AECI-like cells. In an isolated-perfused rat lung model, alveolar fluid reabsorption significantly decreased during high CO2 exposure, which was not prevented by carbonic anhydrase inhibition. Thus, we provide evidence that CAII is expressed in rat alveolar epithelial cells and does not regulate lung alveolar fluid reabsorption during hypercapnia.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Issue number1
StatePublished - Jan 2008


  • Alveolar fluid clearance
  • Carbonic anhydrase II
  • Hypercapnia
  • Intracellular pH
  • Rat alveolar epithelial cells

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


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