Catalase HPI influences membrane permeability in Escherichia coli following near-UV stress

Sara Levén; Amy Heimberger; Abraham Eisenstark

doi:10.1016/0006-291X(90)90816-6

Catalase HPI influences membrane permeability in Escherichia coli following near-UV stress

Sara Levén^*, Amy Heimberger, Abraham Eisenstark

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The katG gene in Escherichia coli encodes catalase HPI, which is involved in membrane transport and protects the cell during oxidative stress. Hydrogen peroxide (H₂O₂) induces synthesis of HPI. We examined the role of HPI in membrane permeability (proline uptake) following exposure to near-ultraviolet radiation (NUV). We found that NUV resulted in the same type of induction as H₂O₂. KatG::Tn10 cells experienced a large drop in uptake after NUV exposure, and levels remained low following incubation. A strain carrying a katG+ plasmid, however, showed considerably less decrease in uptake after NUV, and uptake quickly resumed upon incubation. Further, in an srd mutant which lacks 4-thiouracil, NUV resulted in only a small drop in proline uptake, which was immediately resumed.

Original language	English (US)
Pages (from-to)	1224-1228
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	171
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)90816-6
State	Published - Sep 28 1990
Externally published	Yes

Funding

These investigations were supported by grants from the National Institutes of Health (ES04489), University of Missouri Institutional Research Support Grant RFR 3296 (N.I.H.), and by the Cancer Research Center of Columbia, Missouri. S.L. was an intern under the Howard Hughes Undergraduate Research Program.

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Catalase HPI influences membrane permeability in Escherichia coli following near-UV stress",

abstract = "The katG gene in Escherichia coli encodes catalase HPI, which is involved in membrane transport and protects the cell during oxidative stress. Hydrogen peroxide (H2O2) induces synthesis of HPI. We examined the role of HPI in membrane permeability (proline uptake) following exposure to near-ultraviolet radiation (NUV). We found that NUV resulted in the same type of induction as H2O2. KatG::Tn10 cells experienced a large drop in uptake after NUV exposure, and levels remained low following incubation. A strain carrying a katG+ plasmid, however, showed considerably less decrease in uptake after NUV, and uptake quickly resumed upon incubation. Further, in an srd mutant which lacks 4-thiouracil, NUV resulted in only a small drop in proline uptake, which was immediately resumed.",

author = "Sara Lev{\'e}n and Amy Heimberger and Abraham Eisenstark",

note = "Funding Information: These investigations were supported by grants from the National Institutes of Health (ES04489), University of Missouri Institutional Research Support Grant RFR 3296 (N.I.H.), and by the Cancer Research Center of Columbia, Missouri. S.L. was an intern under the Howard Hughes Undergraduate Research Program.",

year = "1990",

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doi = "10.1016/0006-291X(90)90816-6",

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pages = "1224--1228",

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T1 - Catalase HPI influences membrane permeability in Escherichia coli following near-UV stress

AU - Levén, Sara

AU - Heimberger, Amy

AU - Eisenstark, Abraham

N1 - Funding Information: These investigations were supported by grants from the National Institutes of Health (ES04489), University of Missouri Institutional Research Support Grant RFR 3296 (N.I.H.), and by the Cancer Research Center of Columbia, Missouri. S.L. was an intern under the Howard Hughes Undergraduate Research Program.

PY - 1990/9/28

Y1 - 1990/9/28

N2 - The katG gene in Escherichia coli encodes catalase HPI, which is involved in membrane transport and protects the cell during oxidative stress. Hydrogen peroxide (H2O2) induces synthesis of HPI. We examined the role of HPI in membrane permeability (proline uptake) following exposure to near-ultraviolet radiation (NUV). We found that NUV resulted in the same type of induction as H2O2. KatG::Tn10 cells experienced a large drop in uptake after NUV exposure, and levels remained low following incubation. A strain carrying a katG+ plasmid, however, showed considerably less decrease in uptake after NUV, and uptake quickly resumed upon incubation. Further, in an srd mutant which lacks 4-thiouracil, NUV resulted in only a small drop in proline uptake, which was immediately resumed.

AB - The katG gene in Escherichia coli encodes catalase HPI, which is involved in membrane transport and protects the cell during oxidative stress. Hydrogen peroxide (H2O2) induces synthesis of HPI. We examined the role of HPI in membrane permeability (proline uptake) following exposure to near-ultraviolet radiation (NUV). We found that NUV resulted in the same type of induction as H2O2. KatG::Tn10 cells experienced a large drop in uptake after NUV exposure, and levels remained low following incubation. A strain carrying a katG+ plasmid, however, showed considerably less decrease in uptake after NUV, and uptake quickly resumed upon incubation. Further, in an srd mutant which lacks 4-thiouracil, NUV resulted in only a small drop in proline uptake, which was immediately resumed.

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JO - Biochemical and Biophysical Research Communications

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ER -

Catalase HPI influences membrane permeability in Escherichia coli following near-UV stress

Abstract

Funding

ASJC Scopus subject areas

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