Caveolin-1-eNOS signaling promotes p190RhoGAP-A nitration and endothelial permeability

M. Rizwan Siddiqui, Yulia A. Komarova, Stephen M. Vogel, Xiaopei Gao, Marcelo G. Bonini, Johnson Rajasingh, You Yang Zhao, Viktor Brovkovych, Asrar B. Malik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Endothelial barrier function is regulated by adherens junctions (AJs) and caveolae-mediated transcellular pathways. The opening of AJs that is observed in caveolin-1-/- (Cav-1-/-) endothelium suggests that Cav-1 is necessary for AJ assembly or maintenance. Here, using endothelial cells isolated from Cav-1-/- mice, we show that Cav-1 deficiency induced the activation of endothelial nitric oxide synthase (eNOS) and the generation of nitric oxide (NO) and peroxynitrite. We assessed S-nitrosylation and nitration of AJ-associated proteins to identify downstream NO redox signaling targets. We found that the GTPase-activating protein (GAP) p190RhoGAP-A was selectively nitrated at Tyr1105, resulting in impaired GAP activity and RhoA activation. Inhibition of eNOS or RhoA restored AJ integrity and diminished endothelial hyperpermeability in Cav-1-/- mice. Thrombin, a mediator of increased endothelial permeability, also induced nitration of p120-catenin-associated p190RhoGAP-A. Thus, eNOS-dependent nitration of p190RhoGAP-A represents a crucial mechanism for AJ disassembly and resultant increased endothelial permeability.

Original languageEnglish (US)
Pages (from-to)841-850
Number of pages10
JournalJournal of Cell Biology
Volume193
Issue number5
DOIs
StatePublished - May 30 2011

ASJC Scopus subject areas

  • Cell Biology

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