Cbln1 is essential for interaction-dependent secretion of Cbln3

Dashi Bao, Zhen Pang, Marc A. Morgan, Jennifer Parris, Yongqi Rong, Leyi Li, James I. Morgan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Cbln1 and the orphan glutamate receptor GluRδ2 are pre- and postsynaptic components, respectively, of a novel transneuronal signaling pathway regulating synapse structure and function. We show here that Cbln1 is secreted from cerebellar granule cells in complex with a related protein, Cbln3. However, cbln1- and cbln3-null mice have different phenotypes and cbln1 cbln3 double-null mice have deficits identical to those of cbln1 knockout mice. The basis for these discordant phenotypes is that Cbln1 and Cbln3 reciprocally regulate each other's degradation and secretion such that cbln1-null mice lack both Cbln1 and Cbln3, whereas cbln3-null mice lack Cbln3 but have an approximately sixfold increase in Cbln1. Unlike Cbln1, Cbln3 cannot form homomeric complexes and is secreted only when bound to Cbln1. Structural modeling and mutation analysis reveal that, by constituting a steric clash that is masked upon binding Cbln1 in a "hide-and-run" mechanism of endoplasmic reticulum retention, a single arginine confers the unique properties of Cbln3.

Original languageEnglish (US)
Pages (from-to)9327-9337
Number of pages11
JournalMolecular and cellular biology
Issue number24
StatePublished - Dec 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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