CD34+ cell collection efficiency does not correlate with the preleukapheresis hematocrit

J. Mehta*, Y. Oyama, J. Winter, S. Williams, M. Tallman, S. Singhal, M. Villa, T. Shook, R. Burt, A. Traynor, G. Soff, S. Masarik, G. Ramsey, L. Gordon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

One hundred and seventy-seven large-volume leukapheresis procedures performed on 91 patients over a 15 month period were reviewed to see if the pre-apheresis hematocrit (Hct) affected the CD34+ cell collection efficiency (CE) of the Fenwal CS 3000 Plus cell separator. The Hct was 0.174-0.461 (median 0.317), and the peripheral blood CD34+ cell count 2-2487 per μl (median 21). The total CD34+ cell quantity collected was 3.0-2677.2 × 106 (median 113.0). Based on the number of CD34+ cells contained in the blood volume processed (23.3-37303.2 × 106; median 318.0), the CE was 1.7-87.5% (median 30.3). No correlation was found between the Hct and CE (r2 = 0.0034; P = 0.44) or the total CD34+ cell quantity collected (r2 = 0.0040; P = 0.40). CEs for Hct <0.25 (median CE 36%), Hct 0.25-0.299 (median CE 30%) and Hct 0.30 (median CE 30%) were comparable. As expected, highly significant correlations were seen between the CD34+ cell quantities collected and quantities processed (r2 = 0.59; P < 10-6) as well as the peripheral blood CD34+ cell counts (r2 = 0.60; P < 10-6). We conclude that the minimum acceptable Hct or hemoglobin level for leukapheresis should be dictated by clinical circumstances because it does not affect stem cell collection.

Original languageEnglish (US)
Pages (from-to)597-601
Number of pages5
JournalBone Marrow Transplantation
Volume28
Issue number6
DOIs
StatePublished - 2001

Keywords

  • Apheresis
  • CD34 cells
  • Cell separator
  • Collection efficiency
  • FAHCT
  • Hematocrit
  • Leukapheresis

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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