CD40 ligand trimer enhances the response of CD8+ T cells to Mycobacterium tuberculosis

Buka Samten, Benjamin Wizel, Homayoun Shams, Stephen E. Weis, Peter Klucar, Shiping Wu, Ramakrishna Vankayalapati, Elaine K. Thomas, Satoshi Okada, Alan M. Krensky, Peter F. Barnes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

We investigated the effect of recombinant CD40 ligand trimer (CD40LT) on the functional capacity of peripheral blood CD8+ T cells from healthy tuberculin reactors that were cultured with Mycobacterium tuberculosis-infected autologous monocytes. CD40LT enhanced the capacity of M. tuberculosis-responsive CD8+ T cells to produce IFN-γ by increasing the number of IFN-γ-producing CD8+ T cells and the amount of IFN-γ produced per cell. CD40LT-induced IFN-γ production was dependent on production of IL-12 and IL-18, but did not require IL-15. CD40LT up-regulated expression of the transcription factors phosphorylated CREB and c-Jun, both of which have been previously shown to stimulate IFN-γ mRNA transcription by binding to the IFN-γ promoter. CD40LT also enhanced the capacity of CD8+ T cells to lyse M. tuberculosis-infected monocytes, and increased CTL activity was associated with higher expression of perforin and granulysin, but not of Fas ligand. We conclude that CD40LT can enhance CD8+ T cell effector function in response to M. tuberculosis.

Original languageEnglish (US)
Pages (from-to)3180-3186
Number of pages7
JournalJournal of Immunology
Volume170
Issue number6
DOIs
StatePublished - Mar 15 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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