CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation

A. Bharat, R. C. Fields, N. Steward, E. P. Trulock, G. A. Patterson, T. Mohanakumar*

*Corresponding author for this work

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Chronic human lung allograft rejection is manifested by bronchiolitis obliterans syndrome (BOS). BOS has a multifactorial etiology. Previous studies have indicated that both cellular and humoral alloimmunity play a significant role in the pathogenesis of BOS. Recently, autoimmunity has also been demonstrated to contribute to lung allograft rejection in animal models. However, the significance of autoimmunity in BOS remains unknown. In this report, we investigated the role of naturally occurring CD4+CD25+ regulatory T cells (T-regs) in modulating cellular autoimmunity to collagen type V (col-V), a 'sequestered' yet immunogenic self-protein present in the lung tissue, following lung transplantation (LT). We demonstrated that col-V reactive CD4+ T cells could be detected in the peripheral blood of lung transplant recipients. There was a predominance of IL-10 producing T cells (TIL-10) reactive to col-V with significantly lower levels of IFN-γ and IL-2 producing T cells (Th1 cells). The col-V specific TIL-10 cells suppressed the proliferation and expansion of col-V specific Th1 cells by IL-10-dependent and contact-independent pathways. The TIL-10 cells were distinct but their development was dependent on the presence of T-regs. Furthermore, during chronic lung allograft rejection there was a significant decline of T IL-10 cells with concomitant expansion of col-V-specific IFN-γ producing Th1 cells.

Original languageEnglish (US)
Pages (from-to)1799-1808
Number of pages10
JournalAmerican Journal of Transplantation
Volume6
Issue number8
DOIs
StatePublished - Aug 1 2006

Fingerprint

Collagen Type V
Lung Transplantation
Regulatory T-Lymphocytes
Autoimmunity
Interleukin-10
Bronchiolitis Obliterans
T-Lymphocytes
Th1 Cells
Lung
Allografts
Interleukin-2
Animal Models
Cell Proliferation

Keywords

  • Autoimmunity
  • Bronchiolitis obliterans
  • Collagen type V
  • IL-10
  • Lung transplant
  • Regulatory T cells

ASJC Scopus subject areas

  • Immunology

Cite this

Bharat, A. ; Fields, R. C. ; Steward, N. ; Trulock, E. P. ; Patterson, G. A. ; Mohanakumar, T. / CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation. In: American Journal of Transplantation. 2006 ; Vol. 6, No. 8. pp. 1799-1808.
@article{7239a6d4859a443181b4d08c6b58041c,
title = "CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation",
abstract = "Chronic human lung allograft rejection is manifested by bronchiolitis obliterans syndrome (BOS). BOS has a multifactorial etiology. Previous studies have indicated that both cellular and humoral alloimmunity play a significant role in the pathogenesis of BOS. Recently, autoimmunity has also been demonstrated to contribute to lung allograft rejection in animal models. However, the significance of autoimmunity in BOS remains unknown. In this report, we investigated the role of naturally occurring CD4+CD25+ regulatory T cells (T-regs) in modulating cellular autoimmunity to collagen type V (col-V), a 'sequestered' yet immunogenic self-protein present in the lung tissue, following lung transplantation (LT). We demonstrated that col-V reactive CD4+ T cells could be detected in the peripheral blood of lung transplant recipients. There was a predominance of IL-10 producing T cells (TIL-10) reactive to col-V with significantly lower levels of IFN-γ and IL-2 producing T cells (Th1 cells). The col-V specific TIL-10 cells suppressed the proliferation and expansion of col-V specific Th1 cells by IL-10-dependent and contact-independent pathways. The TIL-10 cells were distinct but their development was dependent on the presence of T-regs. Furthermore, during chronic lung allograft rejection there was a significant decline of T IL-10 cells with concomitant expansion of col-V-specific IFN-γ producing Th1 cells.",
keywords = "Autoimmunity, Bronchiolitis obliterans, Collagen type V, IL-10, Lung transplant, Regulatory T cells",
author = "A. Bharat and Fields, {R. C.} and N. Steward and Trulock, {E. P.} and Patterson, {G. A.} and T. Mohanakumar",
year = "2006",
month = "8",
day = "1",
doi = "10.1111/j.1600-6143.2006.01383.x",
language = "English (US)",
volume = "6",
pages = "1799--1808",
journal = "American Journal of Transplantation",
issn = "1600-6135",
publisher = "Wiley-Blackwell",
number = "8",

}

CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation. / Bharat, A.; Fields, R. C.; Steward, N.; Trulock, E. P.; Patterson, G. A.; Mohanakumar, T.

In: American Journal of Transplantation, Vol. 6, No. 8, 01.08.2006, p. 1799-1808.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation

AU - Bharat, A.

AU - Fields, R. C.

AU - Steward, N.

AU - Trulock, E. P.

AU - Patterson, G. A.

AU - Mohanakumar, T.

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Chronic human lung allograft rejection is manifested by bronchiolitis obliterans syndrome (BOS). BOS has a multifactorial etiology. Previous studies have indicated that both cellular and humoral alloimmunity play a significant role in the pathogenesis of BOS. Recently, autoimmunity has also been demonstrated to contribute to lung allograft rejection in animal models. However, the significance of autoimmunity in BOS remains unknown. In this report, we investigated the role of naturally occurring CD4+CD25+ regulatory T cells (T-regs) in modulating cellular autoimmunity to collagen type V (col-V), a 'sequestered' yet immunogenic self-protein present in the lung tissue, following lung transplantation (LT). We demonstrated that col-V reactive CD4+ T cells could be detected in the peripheral blood of lung transplant recipients. There was a predominance of IL-10 producing T cells (TIL-10) reactive to col-V with significantly lower levels of IFN-γ and IL-2 producing T cells (Th1 cells). The col-V specific TIL-10 cells suppressed the proliferation and expansion of col-V specific Th1 cells by IL-10-dependent and contact-independent pathways. The TIL-10 cells were distinct but their development was dependent on the presence of T-regs. Furthermore, during chronic lung allograft rejection there was a significant decline of T IL-10 cells with concomitant expansion of col-V-specific IFN-γ producing Th1 cells.

AB - Chronic human lung allograft rejection is manifested by bronchiolitis obliterans syndrome (BOS). BOS has a multifactorial etiology. Previous studies have indicated that both cellular and humoral alloimmunity play a significant role in the pathogenesis of BOS. Recently, autoimmunity has also been demonstrated to contribute to lung allograft rejection in animal models. However, the significance of autoimmunity in BOS remains unknown. In this report, we investigated the role of naturally occurring CD4+CD25+ regulatory T cells (T-regs) in modulating cellular autoimmunity to collagen type V (col-V), a 'sequestered' yet immunogenic self-protein present in the lung tissue, following lung transplantation (LT). We demonstrated that col-V reactive CD4+ T cells could be detected in the peripheral blood of lung transplant recipients. There was a predominance of IL-10 producing T cells (TIL-10) reactive to col-V with significantly lower levels of IFN-γ and IL-2 producing T cells (Th1 cells). The col-V specific TIL-10 cells suppressed the proliferation and expansion of col-V specific Th1 cells by IL-10-dependent and contact-independent pathways. The TIL-10 cells were distinct but their development was dependent on the presence of T-regs. Furthermore, during chronic lung allograft rejection there was a significant decline of T IL-10 cells with concomitant expansion of col-V-specific IFN-γ producing Th1 cells.

KW - Autoimmunity

KW - Bronchiolitis obliterans

KW - Collagen type V

KW - IL-10

KW - Lung transplant

KW - Regulatory T cells

UR - http://www.scopus.com/inward/record.url?scp=33745778016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745778016&partnerID=8YFLogxK

U2 - 10.1111/j.1600-6143.2006.01383.x

DO - 10.1111/j.1600-6143.2006.01383.x

M3 - Article

VL - 6

SP - 1799

EP - 1808

JO - American Journal of Transplantation

JF - American Journal of Transplantation

SN - 1600-6135

IS - 8

ER -