CD8 locus nuclear dynamics during thymocyte development

Eleni Ktistaki, Anna Garefalaki, Adam Williams, Simon R. Andrews, Donald M. Bell, Katie E. Foster, Charalampos G. Spilianakis, Richard A. Flavell, Nadezda Kosyakova, Vladmir Trifonov, Thomas Liehr, Dimitris Kioussis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Nuclear architecture and chromatin reorganization have recently been shown to orchestrate gene expression and act as key players in developmental pathways. To investigate how regulatory elements in the mouse CD8 gene locus are arranged in space and in relation to each other, three-dimensional fluorescence in situ hybridization and chromosome conformation capture techniques were employed to monitor the repositioning of the locus in relation to its subchromosomal territory and to identify long-range interactions between the different elements during development. Our data demonstrate that CD8 gene expression in murine lymphocytes is accompanied by the relocation of the locus outside its subchromosomal territory. Similar observations in the CD4 locus point to a rather general phenomenon during T cell development. Furthermore, we show that this relocation of the CD8 gene locus is associated with a clustering of regulatory elements forming a tight active chromatin hub in CD8-expressing cells. In contrast, in nonexpressing cells, the gene remains close to the main body of its chromosomal domain and the regulatory elements appear not to interact with each other.

Original languageEnglish (US)
Pages (from-to)5686-5695
Number of pages10
JournalJournal of Immunology
Issue number10
StatePublished - May 15 2010

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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