Cdk Activity Couples Epigenetic Centromere Inheritance to Cell Cycle Progression

Mariana C C Silva, Dani L. Bodor, Madison E. Stellfox, Nuno M C Martins, Helfrid Hochegger, Daniel R. Foltz, Lars E T Jansen

Research output: Contribution to journalArticlepeer-review

132 Scopus citations


Centromeres form the site of chromosome attachment to microtubules during mitosis. Identity of these loci is maintained epigenetically by nucleosomes containing the histone H3 variant CENP-A. Propagation of CENP-A chromatin is uncoupled from DNA replication initiating only during mitotic exit. We now demonstrate that inhibition of Cdk1 and Cdk2 activities is sufficient to trigger CENP-A assembly throughout the cell cycle in a manner dependent on the canonical CENP-A assembly machinery. We further show that the key CENP-A assembly factor Mis18BP1 HsKNL2 is phosphorylated in a cell cycle-dependent manner that controls its centromere localization during mitotic exit. These results strongly support a model in which the CENP-A assembly machinery is poised for activation throughout the cell cycle but kept in an inactive noncentromeric state by Cdk activity during S, G2, and M phases. Alleviation of this inhibition in G1 phase ensures tight coupling between DNA replication, cell division, and subsequent centromere maturation.

Original languageEnglish (US)
Pages (from-to)52-63
Number of pages12
JournalDevelopmental Cell
Issue number1
StatePublished - Jan 17 2012

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • Cell Biology
  • Developmental Biology


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