cDNA approaches to isolation of the mouse mutant weaver gene

M. E. Hodes; S. R. Dlouhy; J. J. Wei; Y. Wang; L. Sangameswaran; V. Lazar; L. C. Triarhou; B. Ghetti

doi:10.1007/BF00972463

cDNA approaches to isolation of the mouse mutant weaver gene

M. E. Hodes^*, S. R. Dlouhy, J. J. Wei, Y. Wang, L. Sangameswaran, V. Lazar, L. C. Triarhou, B. Ghetti

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

Original language	English (US)
Pages (from-to)	1359-1362
Number of pages	4
Journal	Neurochemical Research
Volume	19
Issue number	11
DOIs	https://doi.org/10.1007/BF00972463
State	Published - Nov 1994

Keywords

Weaver
granule cells
mutant mice
sequence, in situ hybridization

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Biochemistry

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10.1007/BF00972463

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title = "cDNA approaches to isolation of the mouse mutant weaver gene",

abstract = "The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.",

keywords = "Weaver, granule cells, mutant mice, sequence, in situ hybridization",

author = "Hodes, {M. E.} and Dlouhy, {S. R.} and Wei, {J. J.} and Y. Wang and L. Sangameswaran and V. Lazar and Triarhou, {L. C.} and B. Ghetti",

year = "1994",

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AU - Hodes, M. E.

AU - Dlouhy, S. R.

AU - Wei, J. J.

AU - Wang, Y.

AU - Sangameswaran, L.

AU - Lazar, V.

AU - Triarhou, L. C.

AU - Ghetti, B.

PY - 1994/11

Y1 - 1994/11

N2 - The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

AB - The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

KW - Weaver

KW - granule cells

KW - mutant mice

KW - sequence, in situ hybridization

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JO - Neurochemical Research

JF - Neurochemical Research

IS - 11

ER -

cDNA approaches to isolation of the mouse mutant weaver gene

Abstract

Keywords

ASJC Scopus subject areas

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