C/EBPβ regulation of the tumor necrosis factor α gene

Richard M. Pope*, Achim Leutz, Scott A. Ness

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

159 Scopus citations

Abstract

Activated macrophages contribute to chronic inflammation by the secretion of cytokines and proteinases. Tumor necrosis factor α (TNFα) is particularly important in this process because of its ability to regulate other inflammatory mediators in an autocrine and paracrine fashion. The mechanism(s) responsible for the cell type-specific regulation of TNFα is not known. We present data to show that the expression of TNFα is regulated by the transcription factor C/EBPβ (NF-IL6). C/EBPβ activated the TNFα gene promoter in cotransfection assays and bound to it at a site which failed to bind the closely related protein C/EBPα. Finally, a dominant-negative version of C/EBPβ blocked TNFα promoter activation in myeloid cells. Our results implicate C/EBPβ as an important regulator of TNFα by myelomonocytic cells.

Original languageEnglish (US)
Pages (from-to)1449-1455
Number of pages7
JournalJournal of Clinical Investigation
Volume94
Issue number4
DOIs
StatePublished - Oct 1994

Keywords

  • cytokine
  • inflammation
  • promoter
  • transcription factor

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint

Dive into the research topics of 'C/EBPβ regulation of the tumor necrosis factor α gene'. Together they form a unique fingerprint.

Cite this