Cell adhesion to unnatural ligands mediated by a bifunctional protein

Juan Sánchez-Cortés, Katinka Bähr, Milan Mrksich*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

This paper describes a molecular strategy to restore adhesion of cells to surfaces that otherwise do not present ligands that can mediate adhesion. The approach is based on a carbonic anhydrase fusion protein that binds benzenesulfonamides and that also includes the RGD peptide motif that can bind to cell-surface integrin adhesion receptors. In this way, the fusion protein can bind to a monolayer that presents the benzenesulfonamide ligand, thereby positioning the RGD peptide at the surface, where it can mediate the adhesion and spreading of cells. This strategy may provide a general method for promoting the adhesion of cells to non-natural surfaces or to defective biological matrices.

Original languageEnglish (US)
Pages (from-to)9733-9737
Number of pages5
JournalJournal of the American Chemical Society
Volume132
Issue number28
DOIs
StatePublished - Jul 21 2010

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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