TY - JOUR
T1 - Cell Biology
T2 - Kinesin and dynein move a peroxisome in vivo: A tug-of-war or coordinated movement?
AU - Kural, Comert
AU - Kim, Hwajin
AU - Syed, Sheyum
AU - Goshima, Gohta
AU - Gelfand, Vladimir I.
AU - Selvin, Paul R.
PY - 2005/6/3
Y1 - 2005/6/3
N2 - We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ∼8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.
AB - We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ∼8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.
UR - http://www.scopus.com/inward/record.url?scp=20344382542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20344382542&partnerID=8YFLogxK
U2 - 10.1126/science.1108408
DO - 10.1126/science.1108408
M3 - Article
C2 - 15817813
AN - SCOPUS:20344382542
SN - 0036-8075
VL - 308
SP - 1469
EP - 1472
JO - Science
JF - Science
IS - 5727
ER -