Cell Corpse Engulfment Mediated by C. elegans Phosphatidylserine Receptor Through CED-5 and CED-12

Xiaochen Wang, Yi Chun Wu*, Valerie A. Fadok, Ming Chia Lee, Keiko Gengyo-Ando, Li Chun Cheng, Duncan Ledwich, Pei Ken Hsu, Jia Yun Chen, Bin Kuan Chou, Peter Henson, Shohei Mitani, Ding Xue

*Corresponding author for this work

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

During apoptosis, phosphatidylserine, which is normally restricted to the inner leaflet of the plasma membrane, is exposed on the surface of apoptotic cells and has been suggested to act as an "eat-me" signal to trigger phagocytosis. It is unclear how phagocytes recognize phosphatidylserine. Recently, a putative phosphatidylserine receptor (PSR) was identified and proposed to mediate recognition of phosphatidylserine and phagocytosis. We report that psr-1, the Caenorhabditis elegans homolog of PSR, is important for cell corpse engulfment. In vitro PSR-1 binds preferentially phosphatidylserine or cells with exposed phosphatidylserine. In C. elegans, PSR-1 acts in the same cell corpse engulfment pathway mediated by intracellular signaling molecules CED-2 (homologous to the human Crkll protein), CED-5 (DOCK180), CED-10 (Rac GTPase), and CED-12 (ELMO), possibly through direct interaction with CED-5 and CED-12. Our findings suggest that PSR-1 is likely an upstream receptor for the signaling pathway containing CED-2, CED-5, CED-10, and CED-12 proteins and plays an important role in recognizing phosphatidylserine during phagocytosis.

Original languageEnglish (US)
Pages (from-to)1563-1566
Number of pages4
JournalScience
Volume302
Issue number5650
DOIs
StatePublished - Nov 28 2003

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    Wang, X., Wu, Y. C., Fadok, V. A., Lee, M. C., Gengyo-Ando, K., Cheng, L. C., Ledwich, D., Hsu, P. K., Chen, J. Y., Chou, B. K., Henson, P., Mitani, S., & Xue, D. (2003). Cell Corpse Engulfment Mediated by C. elegans Phosphatidylserine Receptor Through CED-5 and CED-12. Science, 302(5650), 1563-1566. https://doi.org/10.1126/science.1087641