Cell cycle-independent induction of D1 and D2 cyclin expression, but not cyclin-Cdk complex formation or Rb phosphorylation, by IFNγ in macrophages

Arunangsu Dey, Wei Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

During G1 to S phase transition, D-type cyclins form complexes with cyclin-dependent kinases (Cdk), which in turn phosphorylate retinoblastoma gene product (Rb) and inhibit its growth-inhibitory function, leading ultimately to cell proliferation. We report here a novel finding that D1 and D2 cyclins are induced in macrophages by antiproliferative factor gamma interferon (IFNγ). The induction appears to be transcriptional activation of the D cyclin genes, since indirect events such as IFNγ-induced colony-stimulating factor-1 (CSF-1) autocrine stimulation, alteration of D1 and D2 mRNA stability and lipopolysaccharide contamination in commercial IFNγ preparations play no roles. In contrast to CSF-1, IFNγ neither induces D1-Cdk4 complex formation and Rb hyperphosphorylation nor interferes with CSF-1-stimulated D1-Cdk4 interaction and Rb phosphorylation, while it completely blocks CSF-1-stimulated cell proliferation. This study suggests that induction of D1 and D2 cyclins is not necessarily associated with cell cycle progression, and D cyclins may have cell cycle-independent functions in response to IFNγ. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)135-147
Number of pages13
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1497
Issue number1
DOIs
StatePublished - Jun 2 2000

Keywords

  • Colony-stimulating factor-1
  • D cyclin
  • Gamma interferon
  • Macrophage
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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