Cell cycle parameters and DNA ploidy in colorectal carcinomas

Naveed Yousuf*, Seema Khan, Yasin Sheikh, David Hyams, Jamal Bokhari, Azra Raza

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Seven patients with colorectal adenocarcinoma and one with squamous cell carcinoma of anorectal region were infused preoperatively with iododeoxyuridine (IUdR) and bromodeoxyuridine (BrdU) in sequence (100 mg/m2 × 1 hr each with 1-hr interval in between) to label S-phase cells. The tumor biopsy specimens were embedded in glycolmethacrylate and 2-μm thick sections were treated with two monoclonal antibodies which permitted the identification of cells which incorporated IUdR only, BrdU only, both IUdR and BrdU, or neither IUdR or BrdU. The labeling index of tumors varied from 17.3 to 35.6% (mean = 25.78 ± 6.162), duration of S-phase ranged from 14.0 to 23.9 hr (mean = 18.73 ± 3.712), and total cell cycle time ranged from 39.4 to 123.4 hr (mean = 76.78 ± 24.165). The architecture of the tumor was well preserved and a variable number of DNA synthesizing mononuclear cells were identified within and around the tumor. Image analysis of Feulgen-stained smears of the tumors was done to measure the DNA content of seven tumor samples. Each tumor was found to be hyperdiploid with multiple modal values. The studies described here demonstrate the feasibility of performing cell cycle kinetic measurements on gastrointestinal tumors which have been labeled in vivo. The ability to perform these measurements on tumor biopsies allows the avoidance of artifacts introduced when solid tumors are disaggregated in vitro for study.

Original languageEnglish (US)
Pages (from-to)457-462
Number of pages6
JournalJournal of Surgical Research
Issue number6
StatePublished - Dec 1991

ASJC Scopus subject areas

  • Surgery


Dive into the research topics of 'Cell cycle parameters and DNA ploidy in colorectal carcinomas'. Together they form a unique fingerprint.

Cite this