Abstract
We introduced a novel approach for the establishment of genetically modified hESC lines, and have shown that mutant hESC may be derived from affected embryos after preimplantation genetic diagnosis (PGD) screening for a particular single gene disorder. Here we describe the procedure of embryo and cell manipulation, their diagnostic layout, and the analysis of the efficiency of embryo development and hESC establishment, as well as the developments for hESC derivation in animal-product-free conditions. Our study shows that a high efficiency of hESC derivation (50%) is especially crucial when working with rare and unique resources such as genetically screened embryos necessary for the derivation of hESC lines that represent specific genetic diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 425-428 |
| Number of pages | 4 |
| Journal | Biophysics |
| Volume | 55 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2010 |
Funding
ACKNOWLEDGMENTS We thank Dr. Koukharenko and Dr. Kuznyetsov for their help; Midwest Fertility Center, IL, Woman and Infants Hospital, RI, for conveying the embryos donated for research. Supported in part by the Illinois Regenerative Medicine Institute and V. Galat’s Eisen berg Scholar Award.
Keywords
- PGD
- Xeno-free
- embryonic stem cells
- human
- single gene disorders
ASJC Scopus subject areas
- Biophysics