@article{1dde1553ff2a4789bdf80ddf5a7450c5,
title = "Cell-free biomanufacturing",
abstract = "Since its early development and use to decipher the genetic code, in vitro or {\textquoteleft}cell-free{\textquoteright} systems have been used as an important research tool to understand biochemical mechanisms and metabolic pathways. More recently, due to important engineering advances the technology is rapidly becoming a biomanufacturing platform for protein therapeutics, vaccines, enzyme biocatalysts, fuels, and commodity chemicals. Here we report recent applications and advances in the cell-free biomanufacturing field and the potential of this emerging approach.",
author = "Bundy, {Bradley C.} and Hunt, {J. Porter} and Jewett, {Michael C.} and Swartz, {James R.} and Wood, {David W.} and Frey, {Douglas D.} and Govind Rao",
note = "Funding Information: This work was supported by National Science Foundation grants CBET-125414ffe8 and DMR-1710574 (to B.C.B), the DARPA Biologically derived Medicines on Demand (Bio-MOD, Arlington, VA) program contract N66001-13-C-4023 (to G.R., D.W.W., D.D.F.), the Army Research Office W911NF-16-1-0372 , W911NF-18-1-0200 , and W911NF1810181 (to M.C.J.), National Science Foundation grants MCB-1413563 and MCB-1716766 (to M.C.J.), the Air Force Research Laboratory Center of Excellence Grant FA8650-15-2-5518 (to M.C.J.), the Defense Threat Reduction Agency Grant HDTRA1-15-10052/P00001 (to M.C.J.), the David and Lucile Packard Foundation (to M.C.J.), the Camille Dreyfus Teacher-Scholar Program (to M.C.J.), the Department of Energy BER Grant DE-SC0018249 (to M.C.J.), EERE Grant DE-EE0008343 (to M.C.J.) and JGI CSP project 503280 and ETOP award (to M.C.J.). The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of Air Force Research Laboratory, Air Force Office of Scientific Research, Defense Threat Reduction Agency, or the U.S. Government. Publisher Copyright: {\textcopyright} 2018",
year = "2018",
month = dec,
doi = "10.1016/j.coche.2018.10.003",
language = "English (US)",
volume = "22",
pages = "177--183",
journal = "Current Opinion in Chemical Engineering",
issn = "2211-3398",
publisher = "Elsevier BV",
}
