Cell-free Biosynthesis of Peptidomimetics

Kanghun Lee, Jessica A. Willi, Namjin Cho, Inseon Kim, Michael C. Jewett*, Joongoo Lee*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations


A wide variety of peptidomimetics (peptide analogs) possessing innovative biological functions have been brought forth as therapeutic candidates through cell-free protein synthesis (CFPS) systems. A key feature of these peptidomimetic drugs is the use of non-canonical amino acid building blocks with diverse biochemical properties that expand functional diversity. Here, we summarize recent technologies leveraging CFPS platforms to expand the reach of peptidomimetic drugs. We also offer perspectives on engineering the translational machinery that may open new opportunities for expanding genetically encoded chemistry to transform drug discovery practice beyond traditional boundaries.

Original languageEnglish (US)
Pages (from-to)905-921
Number of pages17
JournalBiotechnology and Bioprocess Engineering
Issue number6
StatePublished - Dec 2023


  • cell-free protein synthesis systems
  • non-canonical amino acids
  • peptidomimetics
  • translational machinery

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology
  • Biomedical Engineering


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