Cell-free Biosynthesis of Peptidomimetics

Kanghun Lee; Jessica A. Willi; Namjin Cho; Inseon Kim; Michael C. Jewett; Joongoo Lee

doi:10.1007/s12257-022-0268-5

Cell-free Biosynthesis of Peptidomimetics

Kanghun Lee, Jessica A. Willi, Namjin Cho, Inseon Kim, Michael C. Jewett^*, Joongoo Lee^*

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Review article › peer-review

7 Scopus citations

Abstract

A wide variety of peptidomimetics (peptide analogs) possessing innovative biological functions have been brought forth as therapeutic candidates through cell-free protein synthesis (CFPS) systems. A key feature of these peptidomimetic drugs is the use of non-canonical amino acid building blocks with diverse biochemical properties that expand functional diversity. Here, we summarize recent technologies leveraging CFPS platforms to expand the reach of peptidomimetic drugs. We also offer perspectives on engineering the translational machinery that may open new opportunities for expanding genetically encoded chemistry to transform drug discovery practice beyond traditional boundaries.

Original language	English (US)
Pages (from-to)	905-921
Number of pages	17
Journal	Biotechnology and Bioprocess Engineering
Volume	28
Issue number	6
DOIs	https://doi.org/10.1007/s12257-022-0268-5
State	Published - Dec 2023

Funding

This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT, NRF-2021R1C1C1006129) and Ministry of Trade, Industry & Energy (MOTIE, Technology Innovation Program or Industrial Strategic Technology Development Program-Bio-industry technology development program, 20020231; optimization of structure-based mRNA vaccine production and efficacy evaluation). This work was also supported by the Army Research Office (W911NF-16-1-0372; W911NF-18-1-0200), the National Institutes of Health (1U19AI142780-01), and Army Contracting Command (W52P1J-21-9-3023), all to M.C.J.

Keywords

cell-free protein synthesis systems
non-canonical amino acids
peptidomimetics
translational machinery

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-022-0268-5

Cite this

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abstract = "A wide variety of peptidomimetics (peptide analogs) possessing innovative biological functions have been brought forth as therapeutic candidates through cell-free protein synthesis (CFPS) systems. A key feature of these peptidomimetic drugs is the use of non-canonical amino acid building blocks with diverse biochemical properties that expand functional diversity. Here, we summarize recent technologies leveraging CFPS platforms to expand the reach of peptidomimetic drugs. We also offer perspectives on engineering the translational machinery that may open new opportunities for expanding genetically encoded chemistry to transform drug discovery practice beyond traditional boundaries.",

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AU - Willi, Jessica A.

AU - Cho, Namjin

AU - Kim, Inseon

AU - Jewett, Michael C.

AU - Lee, Joongoo

PY - 2023/12

Y1 - 2023/12

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AB - A wide variety of peptidomimetics (peptide analogs) possessing innovative biological functions have been brought forth as therapeutic candidates through cell-free protein synthesis (CFPS) systems. A key feature of these peptidomimetic drugs is the use of non-canonical amino acid building blocks with diverse biochemical properties that expand functional diversity. Here, we summarize recent technologies leveraging CFPS platforms to expand the reach of peptidomimetic drugs. We also offer perspectives on engineering the translational machinery that may open new opportunities for expanding genetically encoded chemistry to transform drug discovery practice beyond traditional boundaries.

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KW - non-canonical amino acids

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KW - translational machinery

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Cell-free Biosynthesis of Peptidomimetics

Abstract

Funding

Keywords

ASJC Scopus subject areas

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