Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes

Filippo Caschera; Jin Woo Lee; Kenneth K.Y. Ho; Allen P. Liu; Michael C. Jewett

doi:10.1039/c6cc00223d

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes

Filippo Caschera, Jin Woo Lee, Kenneth K.Y. Ho, Allen P. Liu^*, Michael C. Jewett

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

Original language	English (US)
Pages (from-to)	5467-5469
Number of pages	3
Journal	Chemical Communications
Volume	52
Issue number	31
DOIs	https://doi.org/10.1039/c6cc00223d
State	Published - 2016

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Chemistry(all)
Catalysis

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10.1039/c6cc00223d

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title = "Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes",

abstract = "A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.",

author = "Filippo Caschera and Lee, {Jin Woo} and Ho, {Kenneth K.Y.} and Liu, {Allen P.} and Jewett, {Michael C.}",

note = "Funding Information: M. C. J. and F. C. gratefully acknowledge the Army Research Office (W911NF-11-1-0445), the David and Lucile Packard Foundation (2011-37152), the Camille Dreyfus Teacher Scholar program, and the Chicago Biomedical Consortium with support from the Searle Funds at the Chicago Community Trust for support. J. W. L. is supported by the NIH's Microfluidics in the Biomedical Sciences Training Program: NIH NIBIB T32 EB005582. A. P. L. and K. K. Y. H. are supported by the NIH Director's New Innovator Award: NIH DP2 HL117748-01. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2016.",

year = "2016",

doi = "10.1039/c6cc00223d",

language = "English (US)",

volume = "52",

pages = "5467--5469",

journal = "Chemical Communications",

issn = "1359-7345",

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T1 - Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with

T2 - In vitro synthesized and assembled ribosomes

AU - Caschera, Filippo

AU - Lee, Jin Woo

AU - Ho, Kenneth K.Y.

AU - Liu, Allen P.

AU - Jewett, Michael C.

N1 - Funding Information: M. C. J. and F. C. gratefully acknowledge the Army Research Office (W911NF-11-1-0445), the David and Lucile Packard Foundation (2011-37152), the Camille Dreyfus Teacher Scholar program, and the Chicago Biomedical Consortium with support from the Searle Funds at the Chicago Community Trust for support. J. W. L. is supported by the NIH's Microfluidics in the Biomedical Sciences Training Program: NIH NIBIB T32 EB005582. A. P. L. and K. K. Y. H. are supported by the NIH Director's New Innovator Award: NIH DP2 HL117748-01. Publisher Copyright: © The Royal Society of Chemistry 2016.

PY - 2016

Y1 - 2016

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AB - A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

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Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes

Abstract

ASJC Scopus subject areas

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