Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes

Filippo Caschera, Jin Woo Lee, Kenneth K.Y. Ho, Allen P. Liu*, Michael C. Jewett

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

Original languageEnglish (US)
Pages (from-to)5467-5469
Number of pages3
JournalChemical Communications
Volume52
Issue number31
DOIs
StatePublished - 2016

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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