Abstract
A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5467-5469 |
| Number of pages | 3 |
| Journal | Chemical Communications |
| Volume | 52 |
| Issue number | 31 |
| DOIs | |
| State | Published - 2016 |
Funding
M. C. J. and F. C. gratefully acknowledge the Army Research Office (W911NF-11-1-0445), the David and Lucile Packard Foundation (2011-37152), the Camille Dreyfus Teacher Scholar program, and the Chicago Biomedical Consortium with support from the Searle Funds at the Chicago Community Trust for support. J. W. L. is supported by the NIH's Microfluidics in the Biomedical Sciences Training Program: NIH NIBIB T32 EB005582. A. P. L. and K. K. Y. H. are supported by the NIH Director's New Innovator Award: NIH DP2 HL117748-01.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Catalysis
- Ceramics and Composites
- General Chemistry
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
