Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes

Filippo Caschera, Jin Woo Lee, Kenneth K.Y. Ho, Allen P. Liu*, Michael C. Jewett

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

Original languageEnglish (US)
Pages (from-to)5467-5469
Number of pages3
JournalChemical Communications
Volume52
Issue number31
DOIs
StatePublished - 2016

Funding

M. C. J. and F. C. gratefully acknowledge the Army Research Office (W911NF-11-1-0445), the David and Lucile Packard Foundation (2011-37152), the Camille Dreyfus Teacher Scholar program, and the Chicago Biomedical Consortium with support from the Searle Funds at the Chicago Community Trust for support. J. W. L. is supported by the NIH's Microfluidics in the Biomedical Sciences Training Program: NIH NIBIB T32 EB005582. A. P. L. and K. K. Y. H. are supported by the NIH Director's New Innovator Award: NIH DP2 HL117748-01.

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Catalysis
  • Ceramics and Composites
  • General Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes'. Together they form a unique fingerprint.

Cite this