TY - JOUR
T1 - Cell-free gene expression
T2 - an expanded repertoire of applications
AU - Silverman, Adam D.
AU - Karim, Ashty S.
AU - Jewett, Michael C.
N1 - Funding Information:
The authors would like to acknowledge members of the Jewett and Lucks Labs for helpful discussions. They also specifically thank R. Murray, J. Swartz, K. Pardee, P. Freemont, J. Collins, and J. Lucks for discussions and input. M.C.J. acknowledges support from the Army Research Office Grants W911NF-16-1-0372, W911NF-19-1-0298 and W911NF-18-1-0200; National Science Foundation Grant MCB-1716766, the Air Force Research Laboratory Center of Excellence Grant FA8650-15-2-5518, the Defense Threat Reduction Agency Grant HDTRA1-15-10052/P00001, the Department of Energy Grant DE-SC0018249, the DOE Joint Genome Institute, the DARPA 1000 Molecules Program HR0011-15-C-0084, the Office of Energy Efficiency and Renewable Energy Grant DE-EE0008343, the Human Frontiers Science Program Grant RGP0015/2017, the National Institutes of Health Grant 1U19AI142780-01, the David and Lucile Packard Foundation, and the Camille Dreyfus Teacher-Scholar Program. A.D.S. was supported in part by the National Institutes of Health Training Grant (T32GM008449) through Northwestern University’s Biotechnology Training Program. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of Air Force Research Laboratory, Air Force Office of Scientific Research, DARPA, Defense Threat Reduction Agency or the U.S. Government.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.
AB - Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.
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U2 - 10.1038/s41576-019-0186-3
DO - 10.1038/s41576-019-0186-3
M3 - Review article
C2 - 31780816
AN - SCOPUS:85076083821
VL - 21
SP - 151
EP - 170
JO - Nature Reviews Genetics
JF - Nature Reviews Genetics
SN - 1471-0056
IS - 3
ER -