Cell-free protein synthesis with prokaryotic combined transcription-translation.

James R. Swartz*, Michael C. Jewett, Kim A. Woodrow

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


Cell-free biology exploits and studies complex biological processes in a controlled environment without intact cells. One model system is prokaryotic cell-free protein synthesis. This technology offers an attractive and convenient approach to produce properly folded recombinant DNA (rDNA) proteins on a laboratory scale, screen PCR fragment libraries in a high-throughput format, express pharmaceutical proteins, incorporate labeled or unnatural amino acids into proteins, and activate microbial physiology to allow for investigation of biological systems. We describe the preparation of materials necessary for the expression, quantification, and purification of rDNA proteins from active Escherichia coli extracts.

Original languageEnglish (US)
Pages (from-to)169-182
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2004

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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