Cell-free systems for accelerating glycoprotein expression and biomanufacturing

Jasmine Hershewe, Weston Kightlinger, Michael C. Jewett*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar moieties, plays critical roles in cellular function, human health, and biotechnology. However, studying and producing defined glycoproteins remains challenging. Cell-free glycoprotein synthesis systems, in which protein synthesis and glycosylation are performed in crude cell extracts, offer new approaches to address these challenges. Here, we review versatile, state-of-the-art systems for biomanufacturing glycoproteins in prokaryotic and eukaryotic cell-free systems with natural and synthetic N-linked glycosylation pathways. We discuss existing challenges and future opportunities in the use of cell-free systems for the design, manufacture, and study of glycoprotein biomedicines.

Original languageEnglish (US)
JournalJournal of Industrial Microbiology and Biotechnology
DOIs
StateAccepted/In press - 2020

Keywords

  • Cell-free protein synthesis
  • Glycoengineering
  • Glycosylation
  • High-throughput experimentation
  • Synthetic biology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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