TY - JOUR
T1 - Cell-free systems for accelerating glycoprotein expression and biomanufacturing
AU - Hershewe, Jasmine
AU - Kightlinger, Weston
AU - Jewett, Michael C.
N1 - Funding Information:
M.C.J. acknowledges support from the Defense Threat Reduction Agency Grant HDTRA1-15-10052/P00001, the DARPA 1000 Molecules Program HR0011-15-C-0084, the Air Force Research Laboratory Center of Excellence Grant FA8650-15-2-5518, the David and Lucile Packard Foundation, and the Camille Dreyfus Teacher-Scholar Program. This project was also supported in part by a fellowship award awarded to J.M.H. through the National Defense Science and Engineering (NDSEG) Fellowship Program, sponsored by the Air Force Research Laboratory, the Office of Naval Research, and the Army Research Office. J.M.H. also received support through the Ryan Fellowship awarded by Northwestern University. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of DARPA, Defense Threat Reduction Agency, or the U.S. Government.
Publisher Copyright:
© 2020, Society for Industrial Microbiology and Biotechnology.
PY - 2020/11
Y1 - 2020/11
N2 - Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar moieties, plays critical roles in cellular function, human health, and biotechnology. However, studying and producing defined glycoproteins remains challenging. Cell-free glycoprotein synthesis systems, in which protein synthesis and glycosylation are performed in crude cell extracts, offer new approaches to address these challenges. Here, we review versatile, state-of-the-art systems for biomanufacturing glycoproteins in prokaryotic and eukaryotic cell-free systems with natural and synthetic N-linked glycosylation pathways. We discuss existing challenges and future opportunities in the use of cell-free systems for the design, manufacture, and study of glycoprotein biomedicines.
AB - Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar moieties, plays critical roles in cellular function, human health, and biotechnology. However, studying and producing defined glycoproteins remains challenging. Cell-free glycoprotein synthesis systems, in which protein synthesis and glycosylation are performed in crude cell extracts, offer new approaches to address these challenges. Here, we review versatile, state-of-the-art systems for biomanufacturing glycoproteins in prokaryotic and eukaryotic cell-free systems with natural and synthetic N-linked glycosylation pathways. We discuss existing challenges and future opportunities in the use of cell-free systems for the design, manufacture, and study of glycoprotein biomedicines.
KW - Cell-free protein synthesis
KW - Glycoengineering
KW - Glycosylation
KW - High-throughput experimentation
KW - Synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85093868428&partnerID=8YFLogxK
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U2 - 10.1007/s10295-020-02321-4
DO - 10.1007/s10295-020-02321-4
M3 - Review article
C2 - 33090335
AN - SCOPUS:85093868428
SN - 1367-5435
VL - 47
SP - 977
EP - 991
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 11
ER -
