Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

Robert M. Silver; Leslie Myatt; John C. Hauth; Kenneth J. Leveno; Alan M. Peaceman; Susan M. Ramin; Philip Samuels; George Saade; Yoram Sorokin; Rebecca G. Clifton; Uma M. Reddy

doi:10.1055/s-0035-1570383

Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

Robert M. Silver^*, Leslie Myatt, John C. Hauth, Kenneth J. Leveno, Alan M. Peaceman, Susan M. Ramin, Philip Samuels, George Saade, Yoram Sorokin, Rebecca G. Clifton, Uma M. Reddy

^*Corresponding author for this work

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

Original language	English (US)
Pages (from-to)	191-198
Number of pages	8
Journal	American journal of perinatology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1055/s-0035-1570383
State	Published - Jan 1 2017

Funding

National Institute of Child Health and Human Development (NICHD) (HD34208, HD27869, HD40485, HD40560, HD40544, HD34116, HD40512, HD21410, HD40545, HD40500, HD27915, HD34136, HD27860, HD53118, HD53097, HD27917, and HD36801); the National Heart, Lung, and Blood Institute; and the National Center for Research Resources (M01 RR00080, UL1 RR024153, UL1 RR024989). Comments and views of the authors do not necessarily represent the views of the NICHD, NHLBI, or NCRR.

Keywords

cell-free DNA
fetal fraction
placenta
preeclampsia

ASJC Scopus subject areas

Obstetrics and Gynecology
Pediatrics, Perinatology, and Child Health

Access to Document

10.1055/s-0035-1570383

Cite this

@article{2478a513524448f58b0279f0a43818c3,

title = "Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia",

abstract = "Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75\%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.",

keywords = "cell-free DNA, fetal fraction, placenta, preeclampsia",

author = "Silver, \{Robert M.\} and Leslie Myatt and Hauth, \{John C.\} and Leveno, \{Kenneth J.\} and Peaceman, \{Alan M.\} and Ramin, \{Susan M.\} and Philip Samuels and George Saade and Yoram Sorokin and Clifton, \{Rebecca G.\} and Reddy, \{Uma M.\}",

note = "Funding Information: National Institute of Child Health and Human Development (NICHD) (HD34208, HD27869, HD40485, HD40560, HD40544, HD34116, HD40512, HD21410, HD40545, HD40500, HD27915, HD34136, HD27860, HD53118, HD53097, HD27917, and HD36801); the National Heart, Lung, and Blood Institute; and the National Center for Research Resources (M01 RR00080, UL1 RR024153, UL1 RR024989). Comments and views of the authors do not necessarily represent the views of the NICHD, NHLBI, or NCRR. Publisher Copyright: {\textcopyright} Copyright 2017 by Thieme Medical Publishers, Inc.",

year = "2017",

month = jan,

day = "1",

doi = "10.1055/s-0035-1570383",

language = "English (US)",

volume = "34",

pages = "191--198",

journal = "American journal of perinatology",

issn = "0735-1631",

publisher = "Thieme Medical Publishers, Inc.",

number = "2",

}

TY - JOUR

T1 - Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

AU - Silver, Robert M.

AU - Myatt, Leslie

AU - Hauth, John C.

AU - Leveno, Kenneth J.

AU - Peaceman, Alan M.

AU - Ramin, Susan M.

AU - Samuels, Philip

AU - Saade, George

AU - Sorokin, Yoram

AU - Clifton, Rebecca G.

AU - Reddy, Uma M.

N1 - Funding Information: National Institute of Child Health and Human Development (NICHD) (HD34208, HD27869, HD40485, HD40560, HD40544, HD34116, HD40512, HD21410, HD40545, HD40500, HD27915, HD34136, HD27860, HD53118, HD53097, HD27917, and HD36801); the National Heart, Lung, and Blood Institute; and the National Center for Research Resources (M01 RR00080, UL1 RR024153, UL1 RR024989). Comments and views of the authors do not necessarily represent the views of the NICHD, NHLBI, or NCRR. Publisher Copyright: © Copyright 2017 by Thieme Medical Publishers, Inc.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

AB - Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

KW - cell-free DNA

KW - fetal fraction

KW - placenta

KW - preeclampsia

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Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

Abstract

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