Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

Robert M. Silver*, Leslie Myatt, John C. Hauth, Kenneth J. Leveno, Alan M Peaceman, Susan M. Ramin, Philip Samuels, George Saade, Yoram Sorokin, Rebecca G. Clifton, Uma M. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalAmerican journal of perinatology
Volume34
Issue number2
DOIs
StatePublished - Jan 1 2017

Fingerprint

First Pregnancy Trimester
Pre-Eclampsia
Mothers
DNA
Serum
Hispanic Americans
African Americans
Body Mass Index
ROC Curve
Case-Control Studies
Antioxidants

Keywords

  • cell-free DNA
  • fetal fraction
  • placenta
  • preeclampsia

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Silver, Robert M. ; Myatt, Leslie ; Hauth, John C. ; Leveno, Kenneth J. ; Peaceman, Alan M ; Ramin, Susan M. ; Samuels, Philip ; Saade, George ; Sorokin, Yoram ; Clifton, Rebecca G. ; Reddy, Uma M. / Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia. In: American journal of perinatology. 2017 ; Vol. 34, No. 2. pp. 191-198.
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title = "Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia",
abstract = "Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75{\%}; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.",
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author = "Silver, {Robert M.} and Leslie Myatt and Hauth, {John C.} and Leveno, {Kenneth J.} and Peaceman, {Alan M} and Ramin, {Susan M.} and Philip Samuels and George Saade and Yoram Sorokin and Clifton, {Rebecca G.} and Reddy, {Uma M.}",
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Silver, RM, Myatt, L, Hauth, JC, Leveno, KJ, Peaceman, AM, Ramin, SM, Samuels, P, Saade, G, Sorokin, Y, Clifton, RG & Reddy, UM 2017, 'Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia', American journal of perinatology, vol. 34, no. 2, pp. 191-198. https://doi.org/10.1055/s-0035-1570383

Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia. / Silver, Robert M.; Myatt, Leslie; Hauth, John C.; Leveno, Kenneth J.; Peaceman, Alan M; Ramin, Susan M.; Samuels, Philip; Saade, George; Sorokin, Yoram; Clifton, Rebecca G.; Reddy, Uma M.

In: American journal of perinatology, Vol. 34, No. 2, 01.01.2017, p. 191-198.

Research output: Contribution to journalArticle

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T1 - Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

AU - Silver, Robert M.

AU - Myatt, Leslie

AU - Hauth, John C.

AU - Leveno, Kenneth J.

AU - Peaceman, Alan M

AU - Ramin, Susan M.

AU - Samuels, Philip

AU - Saade, George

AU - Sorokin, Yoram

AU - Clifton, Rebecca G.

AU - Reddy, Uma M.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

AB - Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia. Study Design Nested case-control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves. Results Cell-free total DNA was higher in African American (median; 25-75%; 6.15; 0.14-28.73; p = 0.02) and Hispanic (4.95; 0.20-26.82; p = 0.037) compared with white women (2.33; 0.03-13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11-25.3) and controls (3.74; 0.12-21.14, p = 0.96). Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

KW - cell-free DNA

KW - fetal fraction

KW - placenta

KW - preeclampsia

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