TY - GEN
T1 - Cell multiplication and movement analysis of swimming Euglena confined in a flow-isolated microaquarium
AU - Ozasa, K.
AU - Lee, J.
AU - Song, S.
AU - Maeda, M.
N1 - Publisher Copyright:
© 14CBMS.
PY - 2014
Y1 - 2014
N2 - We developed a new microchip for cell culture, in which the space for cell culture was isolated from two bypass microfluidic channels for culture medium supply. The chemicals of culture medium permeate from the bypass channels into the flow-isolated micro-aquarium through porous poly-dimethylsiloxane (PDMS) walls. The multiplication of swimming microorganism (Euglena gracilis) from one single cell has been achieved with long-term cell culture more than one month. Unlike other conventional cell-culture microchips, where cells are located in the flow of culture medium, our device is advantageous to culture non-adherent cells including circulating tumor cells, with analyzing cell division, cell movement, or cell differentiation, under fresh culture medium supply.
AB - We developed a new microchip for cell culture, in which the space for cell culture was isolated from two bypass microfluidic channels for culture medium supply. The chemicals of culture medium permeate from the bypass channels into the flow-isolated micro-aquarium through porous poly-dimethylsiloxane (PDMS) walls. The multiplication of swimming microorganism (Euglena gracilis) from one single cell has been achieved with long-term cell culture more than one month. Unlike other conventional cell-culture microchips, where cells are located in the flow of culture medium, our device is advantageous to culture non-adherent cells including circulating tumor cells, with analyzing cell division, cell movement, or cell differentiation, under fresh culture medium supply.
KW - Cell multiplication
KW - Euglena gracilis
KW - Micro-aquarium
KW - PDMS
UR - http://www.scopus.com/inward/record.url?scp=84941670157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941670157&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84941670157
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 497
EP - 499
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -