Cell sedimentation with gravity activation

George Czerlinski*, Robin G Leikin, Debora Reid

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Murine monoclonal antibody T101 has been coupled to thinly polymer-coated heavy alloy particles (LaMn2Ge2). These conjugates are coupled to cultured cells of the human T-cell leukemia line RPMI 8402 (T8402). The sedimentation velocities of cells, of particles, and of cells with particles attached are measured. After determining the mean radii of cells, of particles, and of cells with particles attached, one may compute a mean number of 33 particles attached to a cell. Independently one may compute a mean number of 144 particles/cell for surface saturation. The Appendix handles the underlying theory in three parts: number of particles/cell, saturation number of particles/cell, and resolution for gravity activation. Regarding the latter, cell radii from 4 to 10 μm and particle radii from 0.01 to 1 μm are considered.

Original languageEnglish (US)
Pages (from-to)189-199
Number of pages11
JournalCell Biophysics
Volume13
Issue number3
DOIs
Publication statusPublished - Dec 1 1988

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Keywords

  • cells, sedimentation velocity of
  • Gravity activation
  • human T-cell line 8402
  • surface saturation

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

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