Cell-specific expression of a recombinant rat glutathione S-transferase Ya gene in transgenic mice

C. S. Connelly, W. E. Fahl, T. H. Manoharan, P. M. Iannaccone*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Transgenic mice have been generated which carry a cDNA encoding the rat Ya isozyme of glutathione S-transferase (GST) under the transcriptional control of the SV40 early region promoter-enhancer. Expression of the GST transgene was highly tissue-specific, with the highest expression detected in the convoluted tubular epithelium of the mouse kidney cortex. GST Ya mRNA abundance in these cells was greater than that found for GST Ya mRNA in normal rat liver. GST Ya protein was observed in the convoluted tubule cells of the founder mouse as well as an F1 offspring. The transmission of the foreign gene was followed for two generations, and an erratic pattern of inheritance was observed. These animals provide a model for the in vivo study of GST modulation of carcinogenesis and drug toxicity.

Original languageEnglish (US)
Pages (from-to)7-12
Number of pages6
JournalPathobiology
Volume61
Issue number1
DOIs
StatePublished - 1993

Keywords

  • glutathione S-transferase
  • kidney
  • transgenic mice

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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