Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex

Cathryn R. Cadwell*, Federico Scala, Paul G. Fahey, Dmitry Kobak, Shalaka Mulherkar, Fabian H. Sinz, Stelios Papadopoulos, Zheng H. Tan, Per Johnsson, Leonard Hartmanis, Shuang Li, R. James Cotton, Kimberley F. Tolias, Rickard Sandberg, Philipp Berens, Xiaolong Jiang, Andreas S. Tolias

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Clones of excitatory neurons derived from a common progenitor have been proposed to serve as elementary information processing modules in the neocortex. To characterize the cell types and circuit diagram of clonally related neurons, we performed multi-cell patch clamp recordings and Patch-seq on neurons derived from Nestin-positive progenitors labeled by tamoxifen induction at embryonic day 10.5. The resulting clones are derived from two radial glia on average, span cortical layers 2-6, and are composed of a random sampling of transcriptomic cell types. We find an interaction between shared lineage and connectivity: related neurons are more likely to be connected vertically across cortical layers, but not laterally within the same layer. These findings challenge the view that related neurons show uniformly increased connectivity and suggest that integration of vertical intra-clonal input with lateral inter-clonal input may represent a developmentally programmed connectivity motif supporting the emergence of functional circuits.

Original languageEnglish (US)
JournaleLife
Volume9
DOIs
StatePublished - Mar 2020
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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