@article{2be2f57b230542238641395f6ae451db,
title = "Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex",
abstract = "Clones of excitatory neurons derived from a common progenitor have been proposed to serve as elementary information processing modules in the neocortex. To characterize the cell types and circuit diagram of clonally related neurons, we performed multi-cell patch clamp recordings and Patch-seq on neurons derived from Nestin-positive progenitors labeled by tamoxifen induction at embryonic day 10.5. The resulting clones are derived from two radial glia on average, span cortical layers 2-6, and are composed of a random sampling of transcriptomic cell types. We find an interaction between shared lineage and connectivity: related neurons are more likely to be connected vertically across cortical layers, but not laterally within the same layer. These findings challenge the view that related neurons show uniformly increased connectivity and suggest that integration of vertical intra-clonal input with lateral inter-clonal input may represent a developmentally programmed connectivity motif supporting the emergence of functional circuits.",
author = "Cadwell, {Cathryn R.} and Federico Scala and Fahey, {Paul G.} and Dmitry Kobak and Shalaka Mulherkar and Sinz, {Fabian H.} and Stelios Papadopoulos and Tan, {Zheng H.} and Per Johnsson and Leonard Hartmanis and Shuang Li and Cotton, {R. James} and Tolias, {Kimberley F.} and Rickard Sandberg and Philipp Berens and Xiaolong Jiang and Tolias, {Andreas S.}",
note = "Funding Information: We thank Alexander Ecker, Jacob Reimer, Dimitri Yatsenko, Shan Shen, Emmanouil Froudarakis, Amy Morgan, Camila Lopez, Megan Rech, and Shannon McDonnell for helpful discussions and technical support. We also thank Tomasz Nowakowski for critical reading of the manuscript and for sharing equipment. This project was supported by the Optical Imaging and Vital Microscopy core at Baylor College of Medicine; grants R01MH103108, R01DA028525, DP1EY023176, P30EY002520, T32EY07001, and DP1OD008301 from the National Institutes of Health (NIH) to A.S.T.; grant 707359 from the National Science Foundation to A.S.T.; grants from the Swedish Research Council and the Vallee Foundation to R. S.; grants from the Deutsche Forschungsgemeinschaft (DFG, EXC 2064, BE5601/4-1) and the German Federal Ministry of Education and Research (BMBF; FKZ 01GQ1601) to P.B.; the McKnight Scholar Award to A.S.T.; and the Arnold and Mabel Beckman Foundation Young Investigator Award to A.S.T. X. J. was supported by BCM Faculty Start-up Fund. C.R.C was supported by NIH grants F30MH095440, T32GM007330 and T32EB006350. P.G.F. was supported by NIH grant F30MH112312. C.R.C and P.G.F. were both supported by the Baylor Research Advocates for Student Scientists (BRASS) foundation. This project was also supported by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior/Interior Business Center (DoI/IBC) contract number D16PC00003. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation thereon. Disclaimer: The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of IARPA, DoI/IBC, or the U.S. Government. This project was also supported by the National Institute of Mental Health and the National Institute of Neurological Disorders and Stroke under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: (DFG, EXC 2064, BE5601/4-1) and the German Federal Ministry of Education and Research Funding Information: This project was supported by the Optical Imaging and Vital Microscopy core at Baylor Funding Information: T32EY07001, and DP1OD008301 from the National Institutes of Health (NIH) to A.S.T.; grant Funding Information: Council and the Vallee Foundation to R. S.; grants from the Deutsche Forschungsgemeinschaft Funding Information: This project was also supported by the National Institute of Mental Health and the Funding Information: T32EB006350. P.G.F. was supported by NIH grant F30MH112312. C.R.C and P.G.F. were both Funding Information: Mabel Beckman Foundation Young Investigator Award to A.S.T. X. J. was supported by BCM Funding Information: 707359 from the National Science Foundation to A.S.T.; grants from the Swedish Research Funding Information: This project was also supported by the Intelligence Advanced Research Projects Activity Funding Information: supported by the Baylor Research Advocates for Student Scientists (BRASS) foundation. Funding Information: Faculty Start-up Fund. C.R.C was supported by NIH grants F30MH095440, T32GM007330 and Publisher Copyright: {\textcopyright} 2020, eLife Sciences Publications Ltd. All rights reserved.",
year = "2020",
month = mar,
doi = "10.7554/eLife.52951",
language = "English (US)",
volume = "9",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}