Cell-type-specific GABA responses and chloride homeostasis in the cortex and amygdala

M. Martina, S. Royer, D. Paré*

*Corresponding author for this work

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

The GABA responses of fast-spiking (FS) interneurons and regular-spiking (RS) principal cells were studied using whole cell and perforated-patch recordings in slices of the basolateral amygdala, neo-, and perirhinal cortex. In these three areas, responses to exogenous and synaptically released GABA were abolished by GABAA receptor antagonists in FS cells but also included a GABAB component in RS cells. Moreover, EGABAA of FS and RS cells differed from the calculated ECl (-61 mV), but in opposite direction (FS, -54 mV; RS, -72 mV). This was not due to a differential dialysis of FS and RS cells by the pipette solution because the discrepancy persisted when recordings were obtained with the perforated-patch-clamp technique, using the cationselective ionophore gramicidin. Moreover, pharmacological inhibition of cation-chloride cotransporters revealed that the differing EGABAA of FS and RS neurons arises from cell-type-specific chloride homeostatic mechanisms. Indeed, the prevalent regulators of the intracellular chloride concentration are cotransporters that accumulate chloride in FS cells and extrude chloride in RS neurons. Thus, our results suggest that in the basolateral amygdala as well as in the parietal and perirhinal cortices, FS interneurons are more excitable than principal cells not only by virtue of their dissimilar electroresponsive properties but also because they express a different complement of GABA receptors and chloride homeostatic mechanisms.

Original languageEnglish (US)
Pages (from-to)2887-2895
Number of pages9
JournalJournal of Neurophysiology
Volume86
Issue number6
StatePublished - Dec 27 2001

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Amygdala
gamma-Aminobutyric Acid
Chlorides
Homeostasis
Interneurons
GABA-A Receptor Antagonists
Gramicidin
Neurons
Complement Receptors
Parietal Lobe
GABA Receptors
Ionophores
Patch-Clamp Techniques
Cations
Dialysis
Pharmacology

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

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abstract = "The GABA responses of fast-spiking (FS) interneurons and regular-spiking (RS) principal cells were studied using whole cell and perforated-patch recordings in slices of the basolateral amygdala, neo-, and perirhinal cortex. In these three areas, responses to exogenous and synaptically released GABA were abolished by GABAA receptor antagonists in FS cells but also included a GABAB component in RS cells. Moreover, EGABAA of FS and RS cells differed from the calculated ECl (-61 mV), but in opposite direction (FS, -54 mV; RS, -72 mV). This was not due to a differential dialysis of FS and RS cells by the pipette solution because the discrepancy persisted when recordings were obtained with the perforated-patch-clamp technique, using the cationselective ionophore gramicidin. Moreover, pharmacological inhibition of cation-chloride cotransporters revealed that the differing EGABAA of FS and RS neurons arises from cell-type-specific chloride homeostatic mechanisms. Indeed, the prevalent regulators of the intracellular chloride concentration are cotransporters that accumulate chloride in FS cells and extrude chloride in RS neurons. Thus, our results suggest that in the basolateral amygdala as well as in the parietal and perirhinal cortices, FS interneurons are more excitable than principal cells not only by virtue of their dissimilar electroresponsive properties but also because they express a different complement of GABA receptors and chloride homeostatic mechanisms.",
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Cell-type-specific GABA responses and chloride homeostasis in the cortex and amygdala. / Martina, M.; Royer, S.; Paré, D.

In: Journal of Neurophysiology, Vol. 86, No. 6, 27.12.2001, p. 2887-2895.

Research output: Contribution to journalArticle

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