Cell-type-specific transcription of prospero is controlled by combinatorial signaling in the Drosophila eye

Takashi Hayashi, Chunyan Xu, Richard W. Carthew*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

In Drosophila, Notch and Egfr signaling regulate the determination of many cell types, and yet how these common signals generate cell-specific transcription is not well understood. In the compound eye, prospero (pros) is transcribed specifically in R7 photoreceptors and cone cells. We show that the transcription of pros is activated by two visual-specific transcription selectors, Glass and Sine Oculis, that bind to an enhancer and promote its activation. Together with the pre-patterning transcription factor Lozenge, these factors work in a highly combinatorial manner, such that cells missing any one factor transcribe pros only weakly, if at all. However, the factors are not sufficient to activate the enhancer because of an additional requirement for both Notch and Egfr signals. The loss of Notch signaling produces a 'salt and pepper' effect, with some cells expressing near-normal levels and others expressing no detectable pros at all; thus, the signaling loss does not produce a uniformly reduced level of transcription activity in cells. This suggests a probabilistic mechanism, in which Notch signals influence the likelihood that the enhancer is inactive or fully active in any given cell. The activity level, therefore, is dictated by the proper combination of highly cooperative selector and pre-pattern factors present in the cell.

Original languageEnglish (US)
Pages (from-to)2787-2796
Number of pages10
JournalDevelopment
Volume135
Issue number16
DOIs
StatePublished - Aug 2008

Keywords

  • Drosophila
  • Egfr
  • Notch
  • Photoreceptor

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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