Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation

II. Cellular

Brack G. Hattler, Joshua Miller, Merrill C. Johnson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Mixed lymphocyte culture (MLC) reactivity has been followed in dogs before, during, and after rejection of heterotopically transplanted kidney allografts. During the time of active rejection, there was a loss of cellular reactivity in recipient (R) peripheral blood which could be ascribed to entrapment of immunoreactive cells in the rejecting kidneys. In this period, lymphocytes removed from blood draining the allograft had diminished MLC reactivity when compared to samples obtained from normal venous and arterial blood. Lymphocytes recovered from the rejecting organ demonstrated an accelerated MLC responsiveness to stimulating donor (D) lymphocytes at a time when lymphocytes from peripheral blood had diminished reactivity. Similar reactivity with kidney lymphocytes was also seen using stimulating lymphocytes of indifferent (I) dogs. Following rejection, when MLC responsiveness returned, a state of increased immunity could be demonstrated by an acceleration in the onset of reactivity after incubation in culture for shorter time periods.

Original languageEnglish (US)
Pages (from-to)47-56
Number of pages10
JournalTransplantation
Volume14
Issue number1
DOIs
StatePublished - Jan 1 1972

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Kidney Transplantation
Canidae
Lymphocytes
Kidney
Allografts
Dogs
Immunity

ASJC Scopus subject areas

  • Transplantation

Cite this

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abstract = "Mixed lymphocyte culture (MLC) reactivity has been followed in dogs before, during, and after rejection of heterotopically transplanted kidney allografts. During the time of active rejection, there was a loss of cellular reactivity in recipient (R) peripheral blood which could be ascribed to entrapment of immunoreactive cells in the rejecting kidneys. In this period, lymphocytes removed from blood draining the allograft had diminished MLC reactivity when compared to samples obtained from normal venous and arterial blood. Lymphocytes recovered from the rejecting organ demonstrated an accelerated MLC responsiveness to stimulating donor (D) lymphocytes at a time when lymphocytes from peripheral blood had diminished reactivity. Similar reactivity with kidney lymphocytes was also seen using stimulating lymphocytes of indifferent (I) dogs. Following rejection, when MLC responsiveness returned, a state of increased immunity could be demonstrated by an acceleration in the onset of reactivity after incubation in culture for shorter time periods.",
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Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation : II. Cellular. / Hattler, Brack G.; Miller, Joshua; Johnson, Merrill C.

In: Transplantation, Vol. 14, No. 1, 01.01.1972, p. 47-56.

Research output: Contribution to journalArticle

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