TY - JOUR
T1 - Cellular delivery of MRI contrast agents
AU - Allen, Matthew J.
AU - MacRenaris, Keith W.
AU - Venkatasubramanian, P. N.
AU - Meade, Thomas J.
N1 - Funding Information:
This research is supported by the National Institutes of Health (NIH) Grant Numbers R01 AI47003-03 and 7 R01 AI47003-04 and the Department of Defense Grant Number WSU 02044/DAMD17-02-1-0693. M.J.A. gratefully acknowledges a National Defense Science and Engineering Graduate Fellowship.
PY - 2004/3
Y1 - 2004/3
N2 - Magnetic resonance imaging (MRI) is a powerful tool for acquiring images of opaque living animals with the benefit of tracking events over extended periods of time on the same specimen. Contrast agents are used to enhance regions, tissues, and cells that are magnetically similar but histologically distinct. A principal barrier to the development of MRI contrast agents for investigating biological questions is the delivery of agents across cellular membranes. Here, we describe the synthesis and in vitro testing of Gd(III) -based MRI contrast agents containing varying length polyarginine oligomers capable of permeating cell membranes. We examine the effect of the length of oligomer on T1 enhancement and cellular uptake. Furthermore, the effect of incubation time, concentration, and cell type on uptake is explored. Toxicity and washout studies are performed in addition to MRI phantom studies.
AB - Magnetic resonance imaging (MRI) is a powerful tool for acquiring images of opaque living animals with the benefit of tracking events over extended periods of time on the same specimen. Contrast agents are used to enhance regions, tissues, and cells that are magnetically similar but histologically distinct. A principal barrier to the development of MRI contrast agents for investigating biological questions is the delivery of agents across cellular membranes. Here, we describe the synthesis and in vitro testing of Gd(III) -based MRI contrast agents containing varying length polyarginine oligomers capable of permeating cell membranes. We examine the effect of the length of oligomer on T1 enhancement and cellular uptake. Furthermore, the effect of incubation time, concentration, and cell type on uptake is explored. Toxicity and washout studies are performed in addition to MRI phantom studies.
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U2 - 10.1016/j.chembiol.2004.03.003
DO - 10.1016/j.chembiol.2004.03.003
M3 - Article
C2 - 15123259
AN - SCOPUS:6444229327
SN - 1074-5521
VL - 11
SP - 301
EP - 307
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 3
ER -