Cellular level brain imaging in behaving mammals: An engineering approach

Elizabeth J.O. Hamel; Benjamin F. Grewe; Jones Griffith Parker; Mark J. Schnitzer

doi:10.1016/j.neuron.2015.03.055

Cellular level brain imaging in behaving mammals: An engineering approach

Elizabeth J.O. Hamel, Benjamin F. Grewe, Jones Griffith Parker, Mark J. Schnitzer^*

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Review article › peer-review

118 Scopus citations

Abstract

Fluorescence imaging offers expanding capabilities for recording neural dynamics in behaving mammals, including the means to monitor hundreds of cells targeted by genetic type or connectivity, track cells over weeks, densely sample neurons within local microcircuits, study cells too inactive to isolate in extracellular electrical recordings, and visualize activity in dendrites, axons, or dendritic spines. We discuss recent progress and future directions for imaging in behaving mammals from a systems engineering perspective, which seeks holistic consideration of fluorescent indicators, optical instrumentation, and computational analyses. Today, genetically encoded indicators of neural Ca²⁺ dynamics are widely used, and those of trans-membrane voltage are rapidly improving. Two complementary imaging paradigms involve conventional microscopes for studying head-restrained animals and head-mounted miniature microscopes for imaging in freely behaving animals. Overall, the field has attained sufficient sophistication that increased cooperation between those designing new indicators, light sources, microscopes, and computational analyses would greatly benefit future progress.

Original language	English (US)
Pages (from-to)	140-159
Number of pages	20
Journal	Neuron
Volume	86
Issue number	1
DOIs	https://doi.org/10.1016/j.neuron.2015.03.055
State	Published - Apr 8 2015

Funding

The authors thank B. Ahanonu, M.C. Larkin, Y.Gong, and L.J. Kitch for helpful discussions, D. Dombeck, M.C. Larkin, K. Ghosh, Y. Gong, and Inscopix Inc. for providing figure materials, and J. Marshall, L.J. Kitch, M.C. Larkin, Y. Gong, and J. Lecoq for comments on the manuscript. E.J.O.H. acknowledges the support of a Stanford Graduate Fellowship and the Center for Biomedical Imaging at Stanford. M.J.S. acknowledges research support from NIBIB, NIDA, NINDS, NIMH, NSF, DARPA, the Ellison Foundation, and the Center for Biomedical Imaging at Stanford. M.J.S. is a co-founder and consults scientifically for Inscopix Inc., which has commercialized the miniature integrated microscope technology of Figure 4.

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2015.03.055

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abstract = "Fluorescence imaging offers expanding capabilities for recording neural dynamics in behaving mammals, including the means to monitor hundreds of cells targeted by genetic type or connectivity, track cells over weeks, densely sample neurons within local microcircuits, study cells too inactive to isolate in extracellular electrical recordings, and visualize activity in dendrites, axons, or dendritic spines. We discuss recent progress and future directions for imaging in behaving mammals from a systems engineering perspective, which seeks holistic consideration of fluorescent indicators, optical instrumentation, and computational analyses. Today, genetically encoded indicators of neural Ca2+ dynamics are widely used, and those of trans-membrane voltage are rapidly improving. Two complementary imaging paradigms involve conventional microscopes for studying head-restrained animals and head-mounted miniature microscopes for imaging in freely behaving animals. Overall, the field has attained sufficient sophistication that increased cooperation between those designing new indicators, light sources, microscopes, and computational analyses would greatly benefit future progress.",

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Cellular level brain imaging in behaving mammals: An engineering approach

Abstract

Funding

ASJC Scopus subject areas

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