Cementum structure in Beluga whale teeth

Stuart R Stock*, L. A. Finney, A. Telser, E. Maxey, S. Vogt, J. S. Okasinski

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A large fraction of the volume of Beluga whale (Delphinapterus leucas) teeth consists of cementum, a mineralized tissue which grows throughout the life of the animal and to which the periodontal ligaments attach. Annular growth bands or growth layer groups (GLGs) form within Beluga cementum, and this study investigates GLG structure using X-ray fluorescence mapping and X-ray diffraction mapping with microbeams of synchrotron radiation. The Ca and Zn fluorescent intensities and carbonated hydroxyapatite (cAp) diffracted intensities rise and fall together and match the light-dark bands visible in transmitted light micrographs. Within the bands of maximum Ca and Zn intensity, the ratio of Zn to Ca is slightly higher than in the minima bands. Further, the GLG cAp, Ca and Zn modulation is preserved throughout the cementum for durations >25 year. Statement of significance Cementum is an important tooth tissue to which the periodontal ligaments attach and consists primarily of carbonated apatite mineral and collagen. In optical microscopy of cementum thin sections, light/dark bands are formed annually, and age at death is determined by counting these bands. We employ synchrotron X-ray diffraction and X-ray fluorescence mapping to show the bands in Beluga whale cementum result from differences in mineral content and not from differences in collagen orientation as was concluded by others. Variation in Zn fluorescent intensity was found to be very sensitive indicator of changing biomineralization and suggest that Zn plays an important role this process.

Original languageEnglish (US)
Pages (from-to)289-299
Number of pages11
JournalActa Biomaterialia
Volume48
DOIs
StatePublished - Jan 15 2017

Fingerprint

Beluga Whale
Dental Cementum
Tooth
Ligaments
Collagen
Minerals
Periodontal Ligament
Synchrotrons
Fluorescence
Tissue
Growth
Biomineralization
Light
X-Ray Diffraction
X ray diffraction
X rays
Apatites
Apatite
Durapatite
Synchrotron radiation

Keywords

  • Ca
  • Cementum
  • Dentin
  • Odontocete
  • Synchrotron radiation
  • X-ray diffraction
  • X-ray fluorescence
  • Zn
  • growth layer groups (GLGs)

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Stock, S. R., Finney, L. A., Telser, A., Maxey, E., Vogt, S., & Okasinski, J. S. (2017). Cementum structure in Beluga whale teeth. Acta Biomaterialia, 48, 289-299. https://doi.org/10.1016/j.actbio.2016.11.015
Stock, Stuart R ; Finney, L. A. ; Telser, A. ; Maxey, E. ; Vogt, S. ; Okasinski, J. S. / Cementum structure in Beluga whale teeth. In: Acta Biomaterialia. 2017 ; Vol. 48. pp. 289-299.
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Stock, SR, Finney, LA, Telser, A, Maxey, E, Vogt, S & Okasinski, JS 2017, 'Cementum structure in Beluga whale teeth', Acta Biomaterialia, vol. 48, pp. 289-299. https://doi.org/10.1016/j.actbio.2016.11.015

Cementum structure in Beluga whale teeth. / Stock, Stuart R; Finney, L. A.; Telser, A.; Maxey, E.; Vogt, S.; Okasinski, J. S.

In: Acta Biomaterialia, Vol. 48, 15.01.2017, p. 289-299.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cementum structure in Beluga whale teeth

AU - Stock, Stuart R

AU - Finney, L. A.

AU - Telser, A.

AU - Maxey, E.

AU - Vogt, S.

AU - Okasinski, J. S.

PY - 2017/1/15

Y1 - 2017/1/15

N2 - A large fraction of the volume of Beluga whale (Delphinapterus leucas) teeth consists of cementum, a mineralized tissue which grows throughout the life of the animal and to which the periodontal ligaments attach. Annular growth bands or growth layer groups (GLGs) form within Beluga cementum, and this study investigates GLG structure using X-ray fluorescence mapping and X-ray diffraction mapping with microbeams of synchrotron radiation. The Ca and Zn fluorescent intensities and carbonated hydroxyapatite (cAp) diffracted intensities rise and fall together and match the light-dark bands visible in transmitted light micrographs. Within the bands of maximum Ca and Zn intensity, the ratio of Zn to Ca is slightly higher than in the minima bands. Further, the GLG cAp, Ca and Zn modulation is preserved throughout the cementum for durations >25 year. Statement of significance Cementum is an important tooth tissue to which the periodontal ligaments attach and consists primarily of carbonated apatite mineral and collagen. In optical microscopy of cementum thin sections, light/dark bands are formed annually, and age at death is determined by counting these bands. We employ synchrotron X-ray diffraction and X-ray fluorescence mapping to show the bands in Beluga whale cementum result from differences in mineral content and not from differences in collagen orientation as was concluded by others. Variation in Zn fluorescent intensity was found to be very sensitive indicator of changing biomineralization and suggest that Zn plays an important role this process.

AB - A large fraction of the volume of Beluga whale (Delphinapterus leucas) teeth consists of cementum, a mineralized tissue which grows throughout the life of the animal and to which the periodontal ligaments attach. Annular growth bands or growth layer groups (GLGs) form within Beluga cementum, and this study investigates GLG structure using X-ray fluorescence mapping and X-ray diffraction mapping with microbeams of synchrotron radiation. The Ca and Zn fluorescent intensities and carbonated hydroxyapatite (cAp) diffracted intensities rise and fall together and match the light-dark bands visible in transmitted light micrographs. Within the bands of maximum Ca and Zn intensity, the ratio of Zn to Ca is slightly higher than in the minima bands. Further, the GLG cAp, Ca and Zn modulation is preserved throughout the cementum for durations >25 year. Statement of significance Cementum is an important tooth tissue to which the periodontal ligaments attach and consists primarily of carbonated apatite mineral and collagen. In optical microscopy of cementum thin sections, light/dark bands are formed annually, and age at death is determined by counting these bands. We employ synchrotron X-ray diffraction and X-ray fluorescence mapping to show the bands in Beluga whale cementum result from differences in mineral content and not from differences in collagen orientation as was concluded by others. Variation in Zn fluorescent intensity was found to be very sensitive indicator of changing biomineralization and suggest that Zn plays an important role this process.

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KW - Dentin

KW - Odontocete

KW - Synchrotron radiation

KW - X-ray diffraction

KW - X-ray fluorescence

KW - Zn

KW - growth layer groups (GLGs)

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U2 - 10.1016/j.actbio.2016.11.015

DO - 10.1016/j.actbio.2016.11.015

M3 - Article

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SP - 289

EP - 299

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

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Stock SR, Finney LA, Telser A, Maxey E, Vogt S, Okasinski JS. Cementum structure in Beluga whale teeth. Acta Biomaterialia. 2017 Jan 15;48:289-299. https://doi.org/10.1016/j.actbio.2016.11.015