Centriole biogenesis and function in multiciliated cells

Siwei Zhang, Brian J. Mitchell*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

13 Scopus citations


The use of Xenopus embryonic skin as a model system for the development of ciliated epithelia is well established. This tissue is comprised of numerous cell types, most notably the multiciliated cells (MCCs) that each contain approximately 150 motile cilia. At the base of each cilium lies the centriole-based structure called the basal body. Centriole biogenesis is typically restricted to two new centrioles per cell cycle, each templating from an existing "mother "centriole. In contrast, MCCs are post mitotic cells in which the majority of centrioles arise "de novo "without templating from a mother centriole, instead, these centrioles nucleate from an electron-dense structure termed the deuterostome. How centriole number is regulated in these cells and the mechanism by which the deuterosome templates nascent centrioles is still poorly understood. Here, we describe methods for regulating MCC cell fate as well as for visualizing and manipulating centriole biogenesis.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
Subtitle of host publicationCentrosome
EditorsKaren Oegema, Renata Basto
PublisherAcademic Press Inc
Number of pages25
ISBN (Print)9780128024492
StatePublished - 2015

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X

ASJC Scopus subject areas

  • Cell Biology


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