Centrosome amplification and multinuclear phenotypes are induced by hydrogen peroxide

Sunyoung Chae, Chawon Yun, Haeryun Um, Jae Ho Lee, Hyeseong Cho*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Multinucleated cells resulted from mitosis defect have been noted in pathophysiological states of the cells such as inflammation, senescence and cancer. Since oxidative stress has been known to correlate with these pathophysiological conditions, we tested the effect of H2O 2 on the cell cycle progression and formation of multinucleated cells. H2O2 induced a significant delay in cell cycle progression in Chang liver cells. Interestingly, H2O2 actively induced hyperamplification of centrosomes (n ≥ 3) and multipolar spindle formation during mitosis and subsequently increased the generation of multinucleated cells. A significant increase of the phospho-ERK level was observed upon H2O2 treatment but PD98059, an MEK1/2 inhibitor, didn't reduce the frequency of cells with hyperamplified centrosomes. On the other hand, treatment of either H2O2 or adriamycin increased intracellular ROS levels and multinucleated cells, which were significantly suppressed by antioxidants, N-acetylcysteine and PDTC. Thus, our results suggest that oxidative stress can trigger centrosome hyperamplification and multinucleated cell formation, which may promote pathophysiological progression.

Original languageEnglish (US)
Pages (from-to)482-487
Number of pages6
JournalExperimental and Molecular Medicine
Volume37
Issue number5
DOIs
StatePublished - Oct 31 2005

Keywords

  • Antioxidants
  • Centrosome
  • Giant cells
  • Hydrogen peroxide
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Centrosome amplification and multinuclear phenotypes are induced by hydrogen peroxide'. Together they form a unique fingerprint.

Cite this