Change in mononuclear leukocyte responsiveness in midpregnancy and subsequent preterm birth

Margaret Harper*, Liwu Li, Yuan Zhao, Mark A. Klebanoff, John M. Thorp, Yoram Sorokin, Michael W. Varner, Ronald J. Wapner, Steve N. Caritis, Jay D. Iams, Marshall W. Carpenter, Alan M. Peaceman, Brian M. Mercer, Anthony Sciscione, Dwight J. Rouse, Susan M. Ramin, Garland D. Anderson

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

OBJECTIVE: To estimate the associations of change in immune response with preterm delivery, omega-3 supplementation, and fish diet. METHODS: This was an ancillary study to a randomized trial of omega-3 fatty acid supplementation for the prevention of recurrent preterm birth. In vitro maternal peripheral blood mononuclear leukocyte production of the anti-inflammatory cytokine, interleukin-10, and the proinflammatory cytokine, tumor necrosis factor-α, in response to stimulation with lipopolysaccharide, was measured at 16-22 weeks of gestation (baseline) and again at 25-28 weeks of gestation (follow-up) among women with prior spontaneous preterm birth. Changes in concentrations from baseline to follow-up (Δ) were compared separately among groups defined by gestational age category at delivery, fish diet history, and omega-3 compared with placebo treatment assignment with Kruskal-Wallis tests. RESULTS: Interleukin-10 Δ differed by gestational age category among 292 women with paired assays. Concentrations increased less in women delivering between 35 and 36 6/7 weeks of gestation (48.9 pg/mL) compared with women delivering at term (159.3 pg/mL) and decreased by 65.2 pg/mL in women delivering before 35 weeks of gestation (P=.01). Tumor necrosis factor-α Δ also differed by gestational age category among 319 women, but the pattern was inconsistent. Those delivering between 35 and 36 6/7 weeks of gestation exhibited decreased concentrations of tumor necrosis factor-α at follow-up compared with baseline (2356.0 pg/mL); concentrations increased among women delivering before 35 weeks of gestation and those delivering at term, 132.1 and 86.9 pg/mL (P=.03). Interleukin-10 Δ and tumor necrosis factor-α Δ were unaffected by either omega-3 supplementation or fish diet. CONCLUSION: Recurrent preterm birth was associated with decreased peripheral blood mononuclear leukocyte production of interleukin-10 in response to a stimulus during the second trimester.

Original languageEnglish (US)
Pages (from-to)805-811
Number of pages7
JournalObstetrics and gynecology
Volume121
Issue number4
DOIs
StatePublished - Apr 1 2013

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Mononuclear Leukocytes
Premature Birth
Pregnancy
Interleukin-10
Tumor Necrosis Factor-alpha
Gestational Age
Fishes
Diet
Cytokines
Omega-3 Fatty Acids
Second Pregnancy Trimester
Lipopolysaccharides
Anti-Inflammatory Agents
Placebos
Mothers

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Harper, M., Li, L., Zhao, Y., Klebanoff, M. A., Thorp, J. M., Sorokin, Y., ... Anderson, G. D. (2013). Change in mononuclear leukocyte responsiveness in midpregnancy and subsequent preterm birth. Obstetrics and gynecology, 121(4), 805-811. https://doi.org/10.1097/AOG.0b013e3182878a80
Harper, Margaret ; Li, Liwu ; Zhao, Yuan ; Klebanoff, Mark A. ; Thorp, John M. ; Sorokin, Yoram ; Varner, Michael W. ; Wapner, Ronald J. ; Caritis, Steve N. ; Iams, Jay D. ; Carpenter, Marshall W. ; Peaceman, Alan M. ; Mercer, Brian M. ; Sciscione, Anthony ; Rouse, Dwight J. ; Ramin, Susan M. ; Anderson, Garland D. / Change in mononuclear leukocyte responsiveness in midpregnancy and subsequent preterm birth. In: Obstetrics and gynecology. 2013 ; Vol. 121, No. 4. pp. 805-811.
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abstract = "OBJECTIVE: To estimate the associations of change in immune response with preterm delivery, omega-3 supplementation, and fish diet. METHODS: This was an ancillary study to a randomized trial of omega-3 fatty acid supplementation for the prevention of recurrent preterm birth. In vitro maternal peripheral blood mononuclear leukocyte production of the anti-inflammatory cytokine, interleukin-10, and the proinflammatory cytokine, tumor necrosis factor-α, in response to stimulation with lipopolysaccharide, was measured at 16-22 weeks of gestation (baseline) and again at 25-28 weeks of gestation (follow-up) among women with prior spontaneous preterm birth. Changes in concentrations from baseline to follow-up (Δ) were compared separately among groups defined by gestational age category at delivery, fish diet history, and omega-3 compared with placebo treatment assignment with Kruskal-Wallis tests. RESULTS: Interleukin-10 Δ differed by gestational age category among 292 women with paired assays. Concentrations increased less in women delivering between 35 and 36 6/7 weeks of gestation (48.9 pg/mL) compared with women delivering at term (159.3 pg/mL) and decreased by 65.2 pg/mL in women delivering before 35 weeks of gestation (P=.01). Tumor necrosis factor-α Δ also differed by gestational age category among 319 women, but the pattern was inconsistent. Those delivering between 35 and 36 6/7 weeks of gestation exhibited decreased concentrations of tumor necrosis factor-α at follow-up compared with baseline (2356.0 pg/mL); concentrations increased among women delivering before 35 weeks of gestation and those delivering at term, 132.1 and 86.9 pg/mL (P=.03). Interleukin-10 Δ and tumor necrosis factor-α Δ were unaffected by either omega-3 supplementation or fish diet. CONCLUSION: Recurrent preterm birth was associated with decreased peripheral blood mononuclear leukocyte production of interleukin-10 in response to a stimulus during the second trimester.",
author = "Margaret Harper and Liwu Li and Yuan Zhao and Klebanoff, {Mark A.} and Thorp, {John M.} and Yoram Sorokin and Varner, {Michael W.} and Wapner, {Ronald J.} and Caritis, {Steve N.} and Iams, {Jay D.} and Carpenter, {Marshall W.} and Peaceman, {Alan M.} and Mercer, {Brian M.} and Anthony Sciscione and Rouse, {Dwight J.} and Ramin, {Susan M.} and Anderson, {Garland D.}",
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Harper, M, Li, L, Zhao, Y, Klebanoff, MA, Thorp, JM, Sorokin, Y, Varner, MW, Wapner, RJ, Caritis, SN, Iams, JD, Carpenter, MW, Peaceman, AM, Mercer, BM, Sciscione, A, Rouse, DJ, Ramin, SM & Anderson, GD 2013, 'Change in mononuclear leukocyte responsiveness in midpregnancy and subsequent preterm birth', Obstetrics and gynecology, vol. 121, no. 4, pp. 805-811. https://doi.org/10.1097/AOG.0b013e3182878a80

Change in mononuclear leukocyte responsiveness in midpregnancy and subsequent preterm birth. / Harper, Margaret; Li, Liwu; Zhao, Yuan; Klebanoff, Mark A.; Thorp, John M.; Sorokin, Yoram; Varner, Michael W.; Wapner, Ronald J.; Caritis, Steve N.; Iams, Jay D.; Carpenter, Marshall W.; Peaceman, Alan M.; Mercer, Brian M.; Sciscione, Anthony; Rouse, Dwight J.; Ramin, Susan M.; Anderson, Garland D.

In: Obstetrics and gynecology, Vol. 121, No. 4, 01.04.2013, p. 805-811.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Change in mononuclear leukocyte responsiveness in midpregnancy and subsequent preterm birth

AU - Harper, Margaret

AU - Li, Liwu

AU - Zhao, Yuan

AU - Klebanoff, Mark A.

AU - Thorp, John M.

AU - Sorokin, Yoram

AU - Varner, Michael W.

AU - Wapner, Ronald J.

AU - Caritis, Steve N.

AU - Iams, Jay D.

AU - Carpenter, Marshall W.

AU - Peaceman, Alan M.

AU - Mercer, Brian M.

AU - Sciscione, Anthony

AU - Rouse, Dwight J.

AU - Ramin, Susan M.

AU - Anderson, Garland D.

PY - 2013/4/1

Y1 - 2013/4/1

N2 - OBJECTIVE: To estimate the associations of change in immune response with preterm delivery, omega-3 supplementation, and fish diet. METHODS: This was an ancillary study to a randomized trial of omega-3 fatty acid supplementation for the prevention of recurrent preterm birth. In vitro maternal peripheral blood mononuclear leukocyte production of the anti-inflammatory cytokine, interleukin-10, and the proinflammatory cytokine, tumor necrosis factor-α, in response to stimulation with lipopolysaccharide, was measured at 16-22 weeks of gestation (baseline) and again at 25-28 weeks of gestation (follow-up) among women with prior spontaneous preterm birth. Changes in concentrations from baseline to follow-up (Δ) were compared separately among groups defined by gestational age category at delivery, fish diet history, and omega-3 compared with placebo treatment assignment with Kruskal-Wallis tests. RESULTS: Interleukin-10 Δ differed by gestational age category among 292 women with paired assays. Concentrations increased less in women delivering between 35 and 36 6/7 weeks of gestation (48.9 pg/mL) compared with women delivering at term (159.3 pg/mL) and decreased by 65.2 pg/mL in women delivering before 35 weeks of gestation (P=.01). Tumor necrosis factor-α Δ also differed by gestational age category among 319 women, but the pattern was inconsistent. Those delivering between 35 and 36 6/7 weeks of gestation exhibited decreased concentrations of tumor necrosis factor-α at follow-up compared with baseline (2356.0 pg/mL); concentrations increased among women delivering before 35 weeks of gestation and those delivering at term, 132.1 and 86.9 pg/mL (P=.03). Interleukin-10 Δ and tumor necrosis factor-α Δ were unaffected by either omega-3 supplementation or fish diet. CONCLUSION: Recurrent preterm birth was associated with decreased peripheral blood mononuclear leukocyte production of interleukin-10 in response to a stimulus during the second trimester.

AB - OBJECTIVE: To estimate the associations of change in immune response with preterm delivery, omega-3 supplementation, and fish diet. METHODS: This was an ancillary study to a randomized trial of omega-3 fatty acid supplementation for the prevention of recurrent preterm birth. In vitro maternal peripheral blood mononuclear leukocyte production of the anti-inflammatory cytokine, interleukin-10, and the proinflammatory cytokine, tumor necrosis factor-α, in response to stimulation with lipopolysaccharide, was measured at 16-22 weeks of gestation (baseline) and again at 25-28 weeks of gestation (follow-up) among women with prior spontaneous preterm birth. Changes in concentrations from baseline to follow-up (Δ) were compared separately among groups defined by gestational age category at delivery, fish diet history, and omega-3 compared with placebo treatment assignment with Kruskal-Wallis tests. RESULTS: Interleukin-10 Δ differed by gestational age category among 292 women with paired assays. Concentrations increased less in women delivering between 35 and 36 6/7 weeks of gestation (48.9 pg/mL) compared with women delivering at term (159.3 pg/mL) and decreased by 65.2 pg/mL in women delivering before 35 weeks of gestation (P=.01). Tumor necrosis factor-α Δ also differed by gestational age category among 319 women, but the pattern was inconsistent. Those delivering between 35 and 36 6/7 weeks of gestation exhibited decreased concentrations of tumor necrosis factor-α at follow-up compared with baseline (2356.0 pg/mL); concentrations increased among women delivering before 35 weeks of gestation and those delivering at term, 132.1 and 86.9 pg/mL (P=.03). Interleukin-10 Δ and tumor necrosis factor-α Δ were unaffected by either omega-3 supplementation or fish diet. CONCLUSION: Recurrent preterm birth was associated with decreased peripheral blood mononuclear leukocyte production of interleukin-10 in response to a stimulus during the second trimester.

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