Changes in gene expression associated with loss of function of the NSDHL sterol dehydrogenase in mouse embryonic fibroblasts

David Cunningham, Daniel Swartzlander, Sandya Liyanarachchi, Ramana V. Davuluri, Gail E. Herman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Seven human disorders of postsqualene cholesterol biosynthesis have been described. One of these, congenital hemidysplasia with ichthyosiform nevus and limb defects (CHILD) syndrome, results from mutations in the X-linked gene NADH sterol dehydrogenase-like (NSDHL) encoding a sterol dehydrogenase. A series of mutant alleles of the murine Nsdhl gene are carried by bare patches (Bpa) mice, with Bpa1H representing a null allele. Heterozygous Bpa1H females display skin and skeletal abnormalities in a distribution reflecting random X inactivation, whereas hemizygous male embryos die before embryonic day 10.5. To investigate the molecular basis of defects associated with perturbations in cholesterol biosynthesis, microarray analysis was performed comparing gene expression in embryonic fibroblasts expressing the Bpa 1H allele versus wild-type (wt) cells. Labeled cDNAs from cells grown in normal serum or lipiddepleted serum (LDS) were hybridized to microarrays containing 22,000 mouse genes. Among 44 genes that showed higher expression in the Bpa1H versus wt cells grown in LDS, 11 function in cholesterol biosynthesis, 7 are involved in fatty acid synthesis, 3 ( Srebp2, Insig1, and Orf11) encode sterol-regulatory proteins, and 2 (Ldlrand StarD4) are lipid transporters. Of the 21 remaining genes, 16 are known genes, some of which have been implicated previously in cholesterol homeostasis or lipid-mediated signaling, and 5 are uncharacterized cDNA clones.

Original languageEnglish (US)
Pages (from-to)1150-1162
Number of pages13
JournalJournal of lipid research
Volume46
Issue number6
DOIs
StatePublished - 2005

Keywords

  • Bare patches
  • Cholesterol biosynthesis
  • Congenital hemidysplasia with ichthyosiform nevus and limb defects syndrome
  • Development
  • Microarray
  • Sterol

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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