Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

Joseph R. Moskal*, David A. Gardner, Subhash Basu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Glycolipid glycosyltransferase activities involved in the biosynthesis in vitro of neutral and acidic glycosphingolipids were measured in C-1300 tumors and cloned cells derived therefrom. An adrenergic clone (NIE-115) was grown in tissue culture in the presence of dibutyryl cyclic AMP and the levels of glycosyltransferases were measured before and after differentiation. Increased activities of galactosyltransferases and sialyl-transferases with a concomitant increase in glutamate decarboxylase activity (the enzyme that catalyzes the synthesis of an inhibitory neurotransmitter, γ-aminobutyric acid) were observed.

Original languageEnglish (US)
Pages (from-to)751-758
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number2
DOIs
StatePublished - Nov 27 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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