Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

Joseph R. Moskal; David A. Gardner; Subhash Basu

doi:10.1016/0006-291X(74)91021-3

Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

Joseph R. Moskal^*, David A. Gardner, Subhash Basu

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Glycolipid glycosyltransferase activities involved in the biosynthesis in vitro of neutral and acidic glycosphingolipids were measured in C-1300 tumors and cloned cells derived therefrom. An adrenergic clone (NIE-115) was grown in tissue culture in the presence of dibutyryl cyclic AMP and the levels of glycosyltransferases were measured before and after differentiation. Increased activities of galactosyltransferases and sialyl-transferases with a concomitant increase in glutamate decarboxylase activity (the enzyme that catalyzes the synthesis of an inhibitory neurotransmitter, γ-aminobutyric acid) were observed.

Original language	English (US)
Pages (from-to)	751-758
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(74)91021-3
State	Published - Nov 27 1974

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells",

abstract = "Glycolipid glycosyltransferase activities involved in the biosynthesis in vitro of neutral and acidic glycosphingolipids were measured in C-1300 tumors and cloned cells derived therefrom. An adrenergic clone (NIE-115) was grown in tissue culture in the presence of dibutyryl cyclic AMP and the levels of glycosyltransferases were measured before and after differentiation. Increased activities of galactosyltransferases and sialyl-transferases with a concomitant increase in glutamate decarboxylase activity (the enzyme that catalyzes the synthesis of an inhibitory neurotransmitter, γ-aminobutyric acid) were observed.",

author = "Moskal, {Joseph R.} and Gardner, {David A.} and Subhash Basu",

note = "Funding Information: was supported Miles Laboratories, in the Chemistry the grant NS-09541",

year = "1974",

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doi = "10.1016/0006-291X(74)91021-3",

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T1 - Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

AU - Moskal, Joseph R.

AU - Gardner, David A.

AU - Basu, Subhash

N1 - Funding Information: was supported Miles Laboratories, in the Chemistry the grant NS-09541

PY - 1974/11/27

Y1 - 1974/11/27

N2 - Glycolipid glycosyltransferase activities involved in the biosynthesis in vitro of neutral and acidic glycosphingolipids were measured in C-1300 tumors and cloned cells derived therefrom. An adrenergic clone (NIE-115) was grown in tissue culture in the presence of dibutyryl cyclic AMP and the levels of glycosyltransferases were measured before and after differentiation. Increased activities of galactosyltransferases and sialyl-transferases with a concomitant increase in glutamate decarboxylase activity (the enzyme that catalyzes the synthesis of an inhibitory neurotransmitter, γ-aminobutyric acid) were observed.

AB - Glycolipid glycosyltransferase activities involved in the biosynthesis in vitro of neutral and acidic glycosphingolipids were measured in C-1300 tumors and cloned cells derived therefrom. An adrenergic clone (NIE-115) was grown in tissue culture in the presence of dibutyryl cyclic AMP and the levels of glycosyltransferases were measured before and after differentiation. Increased activities of galactosyltransferases and sialyl-transferases with a concomitant increase in glutamate decarboxylase activity (the enzyme that catalyzes the synthesis of an inhibitory neurotransmitter, γ-aminobutyric acid) were observed.

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Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

Abstract

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