This chapter discusses the uses of enucleated cells. The enucleation phenomenon appears to be related to the response of cells to the drug at concentrations of 5–10 μg/ml. At these concentrations, the cells have a grossly altered morphology and the nucleus is raised up from the growth substrate onto a slender stalk of cytoplasm. In a few cases this stalk breaks and the nucleus is released from the cells, apparently surrounded by a thin sheet of cytoplasm and a plasma membrane. With more than l06 enucleates easily obtainable in one cycle of centrifugation it has become possible to study the biochemistry as well as the physiology of cytoplasm. Enucleation with cytochalasin B does not result in irreversible damage to cultured cells, and the normality of these cytoplasms is rather surprising. For at least 12 hours, enucleates are viable by such disparate criteria as cell movement, virus replication, and protein synthesis. They serve as an excellent system for a broad spectrum of studies on nuclear-cytoplasmic interactions in eukaryotic cells.
ASJC Scopus subject areas
- Cell Biology