Chapter Four Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex

Christine Feig*, Marcus E. Peter

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Major work elucidating the structure and function of the CD95 death inducing signaling complex (DISC) was carried in the late 1990s. Since then the DISC has become a paradigm for multiprotein signaling complexes in the apoptosis literature. We analyzed the earliest events of DISC formation with a set of different techniques and found a surprising new characteristic of the CD95 DISC. Data revealed that CD95 is palmitoylated on cysteine 199. This lipid modification enhances receptor aggregation to the high molecular weight DISC.

Original languageEnglish (US)
Title of host publicationProgrammed Cell Death,General Principles forStudying Cell Death, Part A
EditorsRoya Khosravi-Far, Zahra Zakeri, Richard Lockshin, Mauro Piacentini
Pages83-100
Number of pages18
DOIs
StatePublished - Sep 24 2008

Publication series

NameMethods in Enzymology
Volume442
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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