Chapter Four Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex

Christine Feig; Marcus E. Peter

doi:10.1016/S0076-6879(08)01404-3

Chapter Four Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex

Christine Feig^*, Marcus E. Peter

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Major work elucidating the structure and function of the CD95 death inducing signaling complex (DISC) was carried in the late 1990s. Since then the DISC has become a paradigm for multiprotein signaling complexes in the apoptosis literature. We analyzed the earliest events of DISC formation with a set of different techniques and found a surprising new characteristic of the CD95 DISC. Data revealed that CD95 is palmitoylated on cysteine 199. This lipid modification enhances receptor aggregation to the high molecular weight DISC.

Original language	English (US)
Title of host publication	Programmed Cell Death,General Principles forStudying Cell Death, Part A
Editors	Roya Khosravi-Far, Zahra Zakeri, Richard Lockshin, Mauro Piacentini
Pages	83-100
Number of pages	18
DOIs	https://doi.org/10.1016/S0076-6879(08)01404-3
State	Published - 2008

Publication series

Name	Methods in Enzymology
Volume	442
ISSN (Print)	0076-6879

Funding

The work was supported by the National Institutes of Health (R01 CA93519).

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0076-6879(08)01404-3

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Feig, C., & Peter, M. E. (2008). Chapter Four Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex. In R. Khosravi-Far, Z. Zakeri, R. Lockshin, & M. Piacentini (Eds.), Programmed Cell Death,General Principles forStudying Cell Death, Part A (pp. 83-100). (Methods in Enzymology; Vol. 442). https://doi.org/10.1016/S0076-6879(08)01404-3

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abstract = "Major work elucidating the structure and function of the CD95 death inducing signaling complex (DISC) was carried in the late 1990s. Since then the DISC has become a paradigm for multiprotein signaling complexes in the apoptosis literature. We analyzed the earliest events of DISC formation with a set of different techniques and found a surprising new characteristic of the CD95 DISC. Data revealed that CD95 is palmitoylated on cysteine 199. This lipid modification enhances receptor aggregation to the high molecular weight DISC.",

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Chapter Four Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex. / Feig, Christine; Peter, Marcus E.
Programmed Cell Death,General Principles forStudying Cell Death, Part A. ed. / Roya Khosravi-Far; Zahra Zakeri; Richard Lockshin; Mauro Piacentini. 2008. p. 83-100 (Methods in Enzymology; Vol. 442).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AB - Major work elucidating the structure and function of the CD95 death inducing signaling complex (DISC) was carried in the late 1990s. Since then the DISC has become a paradigm for multiprotein signaling complexes in the apoptosis literature. We analyzed the earliest events of DISC formation with a set of different techniques and found a surprising new characteristic of the CD95 DISC. Data revealed that CD95 is palmitoylated on cysteine 199. This lipid modification enhances receptor aggregation to the high molecular weight DISC.

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Chapter Four Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex

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