Characteristics of voltage sensitive calcium channels in dendrites of cultured rat cerebellar neurons

Vytautas P. Bindokas, James R. Brorson, Richard J. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We examined the effects of various pharmacological agents on the Ca2+ channels existing in the dendrites of cultured rat cerebellar neurons. The cultures consisted of Purkinje cells and non-Purkinje cells (deep cerebellar nuclear neurons and other non-granule neurons). Changes in intracellular free calcium concentration, [Ca2+]i, were monitored by digital imaging microfluorimetry using fura-2 as the indicator dye. In the Purkinje cell population increases in dendritic [Ca2+]i evoked by brief pulses of high K+ were very effectively blocked by (> 80%) by ω-Aga-IVA at low concentrations. Nimodipine and ω-conotoxins GVIA and MVIIC only blocked small components of the [Ca2+]i rise. In the non-Purkinje cells, ω-Aga-IVA was much less effective. ω-Conotoxin-GVIA blocked somewhat more and nimodipine blocked a similar percentage of the [Ca2+]i rise. ω-Conotoxin-MVIIC was quite ineffective in these cells. The results are discussed in terms of the types of voltage sensitive Ca2+ channels existing in the dendrites of these cells.

Original languageEnglish (US)
Pages (from-to)1213-1220
Number of pages8
JournalNeuropharmacology
Volume32
Issue number11
DOIs
StatePublished - Nov 1993

Keywords

  • Purkinje neurons
  • ω-Agatoxin-IVA
  • ω-conotoxin-GVIA
  • ω-conotoxin-MVIIC

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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