Characterization of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific germinal center B cells and antigen-binding B220- cells after primary NP challenge in mice

Kristy L. Wolniak, Randolph J. Noelle, Thomas J. Waldschmidt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Previous studies examining the primary germinal center (GC) response to SRBC in mice demonstrated a steady ratio of IgM+ to isotype-switched GC B cells and a persistent population of GC B cells with a founder phenotype. These characteristics held true at the inductive, plateau, and dissociative phases of the GC response, suggesting a steady-state environment. To test whether these characteristics apply to the primary response of other T cell-dependent Ags, the present study examined the GC response after challenge with (4-hydroxy-3-nitrophenyl)acetyl (NP) in C57BL/6 mice. Multiparameter flow cytometric analysis was used to assess the phenotype of splenic NP-reactive cells at multiple time points after immunization. Results of these studies demonstrated the characteristics of the SRBC-induced GC reaction to be fully maintained in the NP response. In particular, there was a steady ratio of nonswitched to switched B cells, with the majority of NP-reactive GC B cells displaying IgM. In addition, a substantial frequency of B220- NP-binding cells was observed in the spleen at later time points after NP challenge. Although these cells were IgE+, they were found to express both κ and λ L chains and display the high-affinity IgE Fc (FcεRI) receptor, suggesting that this population is not of B cell origin. Adoptive transfer studies further demonstrated the B220- NP-binding subset to be derived from the myeloid lineage.

Original languageEnglish (US)
Pages (from-to)2072-2079
Number of pages8
JournalJournal of Immunology
Issue number4
StatePublished - Aug 15 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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