Characterization of a novel myeloma cell line, MM.1

R. E. Goldman-Leikin, H. R. Salwen, C. V. Herst, D. Variakojis, M. L. Bian, M. M. Le Beau, P. Selvanayagan, R. Marder, R. Anderson, S. Weitzman, S. T. Rosen

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Abstract

A myeloma cell line, MM.1, has been established from the peripheral blood cells of a patient with immunoglobulin A myeloma. MM.1 grows in suspension either singly or in small clusters and secretes λ-light chain. Phenotypically, MM.1 cells lack most B cell antigens, but they do express human leukocyte antigen DR, PCA-1, and T9 and T10 antigens. Molecular analysis of MM.1 demonstrates that it is negative for the presence of the Epstein-Barr virus genome. Southern analysis of MM.1 detected a rearrangement of the λ-light chain gene, and Northern blot analysis revealed high levels of λ gene expression. Cytogenetic analysis of the MM.1 cell line revealed the presence of seven related chromosomally abnormal cell lines characterized by numerical and structural aberrations, and it revealed five nonclonal abnormal cells. The most notable abnormality is a reciprocal translocation involving band q24.3 of chromosome 12 and band q32.3 of chromosome 14; translocations involving 14q32 are frequently observed in neoplasms of B cell origin.

Original languageEnglish (US)
Pages (from-to)335-345
Number of pages11
JournalJournal of Laboratory and Clinical Medicine
Volume113
Issue number3
Publication statusPublished - Jan 1 1989

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Goldman-Leikin, R. E., Salwen, H. R., Herst, C. V., Variakojis, D., Bian, M. L., Le Beau, M. M., ... Rosen, S. T. (1989). Characterization of a novel myeloma cell line, MM.1. Journal of Laboratory and Clinical Medicine, 113(3), 335-345.