Characterization of a rat insulin-like growth factor I gene promoter

William L. Lowe*, Rebecca M. Teasdale

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Rat IGF-I mRNAs contain one of two alternative 5′-untranslated regions which are encoded by alternative exons (exons 1 and 2) and whose expression is controlled by alternative promoter elements. We investigated the ability of fragments of DNA which contain exon 1 and its 5′-flanking region to regulate transcription of a luciferase reporter gene in transient transfection assays. Maximal promoter activity was obtained with a construct which contained 412 bp of 5′-flanking region while constructs which contained 1120 and 1690 bp of 5′-flanking region induced ∼50% less enzymatic activity. Mapping of transcription start sites by RNase protection assay demonstrated that native start sites were used by these constructs, although the relative use of different start sites was different from start site usage by the endogenous gene. These data demonstrate that the 5′-flanking region of exon 1 is capable of regulating transcription of IGF-I mRNAs.

Original languageEnglish (US)
Pages (from-to)972-978
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume189
Issue number2
DOIs
StatePublished - Dec 15 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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