TY - JOUR
T1 - Characterization of an antigen in acute anaphylactic dialysis reactions
T2 - Ethylene oxide-altered human serum albumin
AU - Grammer, Leslie C.
AU - Shaughnessy, Martha A.
AU - Paterson, Bruce F.
AU - Patterson, Roy
N1 - Funding Information:
From the Section of Allergy and Immunology, Department of Med-icine, Northwestern University Medical School. Chicago, Ill. Supported by United States Public Health Service Grant AI-11403 and the Ernest S. Bazley Trust. Received for publication Oct. IO, 1984. Accepted for publication March 12, 1985. Reprint requests: Leslie C. Grammer. M.D., Northwestern Unl-versity Medical School, 303 E. Chicago Ave.. Chicago, IL 60611.
PY - 1985/11
Y1 - 1985/11
N2 - Anaphylactic reactions to dialysis have been associated with antibody to human serum albumin (HSA) that has been reacted with ethylene oxide (ETO). ETO, an agent used to sterilize dialyzers, is a potent alkylator of proteins. We have studied the antigenic characteristics of ETO-HSA. By use of immunoelectrophoresis we have demonstrated differences in electrophoretic mobility between HSA and ETO-HSA. By gel chromatography we have observed an apparent increase in the molecular weight of ETO-HSA as compared to HSA. Antigenic activity of ETO-HSA as determined by the technique of Lidd and Farr is found in all molecular weight fractions. Cutaneous reactivity to ETO-HSA can be transferred to subhuman primates. That reactivity is lost if the sera are heated to 56 °C for 4 hours. Antibody activity to ETO-HSA cannot be inhibited by ETO-glycine or HSA but can partially be inhibited by ETO-polylysine or ETO-ovalbumin. This suggests that antibody is directed against a new antigenic determinant on the ETO-protein moiety, probably an ETO-amino acid determinant like lysine.
AB - Anaphylactic reactions to dialysis have been associated with antibody to human serum albumin (HSA) that has been reacted with ethylene oxide (ETO). ETO, an agent used to sterilize dialyzers, is a potent alkylator of proteins. We have studied the antigenic characteristics of ETO-HSA. By use of immunoelectrophoresis we have demonstrated differences in electrophoretic mobility between HSA and ETO-HSA. By gel chromatography we have observed an apparent increase in the molecular weight of ETO-HSA as compared to HSA. Antigenic activity of ETO-HSA as determined by the technique of Lidd and Farr is found in all molecular weight fractions. Cutaneous reactivity to ETO-HSA can be transferred to subhuman primates. That reactivity is lost if the sera are heated to 56 °C for 4 hours. Antibody activity to ETO-HSA cannot be inhibited by ETO-glycine or HSA but can partially be inhibited by ETO-polylysine or ETO-ovalbumin. This suggests that antibody is directed against a new antigenic determinant on the ETO-protein moiety, probably an ETO-amino acid determinant like lysine.
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U2 - 10.1016/0091-6749(85)90669-4
DO - 10.1016/0091-6749(85)90669-4
M3 - Article
C2 - 3932500
AN - SCOPUS:0022179179
SN - 0091-6749
VL - 76
SP - 670
EP - 675
JO - The Journal of allergy and clinical immunology
JF - The Journal of allergy and clinical immunology
IS - 5
ER -