TY - JOUR
T1 - Characterization of elastin protein and mRNA from salmonid fish (Oncorhynchus kisutch)
AU - Chow, Marguerite
AU - Boyd, Charles D.
AU - Iruela-Arispe, Maria Luisa
AU - Wrenn, David S.
AU - Mecham, Robert
AU - Helene Sage, E.
PY - 1989
Y1 - 1989
N2 - 1. 1. Elastin was isolated from the bulbus arteriosus of a salmonid fish. Monoclonal and polyclonal antibodie,s elicited against a CNBr digest of this protein, immunoprecipitated a polypeptide of Mr 43,000 from fish cell culture medium. 2. 2. Cell-free translation of salmon poly A+ RNA produced a protein of approximately 43 kD that was immunoprecipitated with anti-elastin antibodies. The corresponding mRNA had an approximate Mr of 2 kb. 3. 3. Despite similarities in amino acid composition, the differences in Mr between mammalian and salmon mRNA and protein suggest a divergence of fish and higher vertebrate elastins from an earlier ancestral gene.
AB - 1. 1. Elastin was isolated from the bulbus arteriosus of a salmonid fish. Monoclonal and polyclonal antibodie,s elicited against a CNBr digest of this protein, immunoprecipitated a polypeptide of Mr 43,000 from fish cell culture medium. 2. 2. Cell-free translation of salmon poly A+ RNA produced a protein of approximately 43 kD that was immunoprecipitated with anti-elastin antibodies. The corresponding mRNA had an approximate Mr of 2 kb. 3. 3. Despite similarities in amino acid composition, the differences in Mr between mammalian and salmon mRNA and protein suggest a divergence of fish and higher vertebrate elastins from an earlier ancestral gene.
UR - http://www.scopus.com/inward/record.url?scp=0024377643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024377643&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(89)90055-2
DO - 10.1016/0305-0491(89)90055-2
M3 - Article
C2 - 2805642
AN - SCOPUS:0024377643
SN - 0305-0491
VL - 93
SP - 835
EP - 845
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
IS - 4
ER -