Characterization of elastin protein and mRNA from salmonid fish (Oncorhynchus kisutch)

Marguerite Chow, Charles D. Boyd, Maria Luisa Iruela-Arispe, David S. Wrenn, Robert Mecham, E. Helene Sage*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

1. 1. Elastin was isolated from the bulbus arteriosus of a salmonid fish. Monoclonal and polyclonal antibodie,s elicited against a CNBr digest of this protein, immunoprecipitated a polypeptide of Mr 43,000 from fish cell culture medium. 2. 2. Cell-free translation of salmon poly A+ RNA produced a protein of approximately 43 kD that was immunoprecipitated with anti-elastin antibodies. The corresponding mRNA had an approximate Mr of 2 kb. 3. 3. Despite similarities in amino acid composition, the differences in Mr between mammalian and salmon mRNA and protein suggest a divergence of fish and higher vertebrate elastins from an earlier ancestral gene.

Original languageEnglish (US)
Pages (from-to)835-845
Number of pages11
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume93
Issue number4
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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